Purified anti-Neurogranin, 70-78 Antibody

Pricing & Availability
Clone
A16007A (See other available formats)
Other Names
NRGN, Ng, RC3, Calmodulin-Binding Protein, Protein Kinase C Substrate, Hng
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16007A_PURE_Neurogranin_70-78_Antibody_1_IHC_010417
IHC staining of purified anti-Neurogranin antibody (clone A16007A) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol.
  • A16007A_PURE_Neurogranin_70-78_Antibody_1_IHC_010417
    IHC staining of purified anti-Neurogranin antibody (clone A16007A) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 5 µg/mL overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol.
  • A16007A_PURE_Neurogranin_70-78_Antibody_2_WB_010517
    Western blot of purified anti-Neurogranin antibody (clone A16007A) and isotype-matched IgG2b control, demonstrating antibody specificity towards endogenous neurogranin protein. Lane 1: 20 µg of human brain tissue lysate; Lane 2: 20 µg of human Alzheimer's disease brain tissue lysate; Lanes 3 and 4: 5 µg of rat brain tissue lysate; Lane 5: 50 ng of human recombinant Neurogranin-GST protein. Blots were incubated with 1 µg/mL of the primary antibody overnight at 4°C followed by incubation with HRP labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
See high resolution IHC-P/WB data...
Cat # Size Price Quantity Avail. Save
848501 25 µg $90
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848502 100 µg $225
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Description

Neurogranin (NRGN) is the main post-synaptic protein kinase substrate that binds to calmodulin (CaM) in the absence of calcium. It is suggested that NRGN is a direct target for thyroid hormone in the human brain, and that the control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. Neurogranin in CSF has been implicated as a possible marker for synaptic degeneration in Alzheimer’s disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated synthetic peptide corresponding to amino acid residues 70-78 of human neurogranin protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ELISA Capture, IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650691 (BioLegend Cat. No. 848501)
AB_2650692 (BioLegend Cat. No. 848502)

Antigen Details

Structure
Neurogranin is a 78 amino acid protein with a molecular mass of approximately 7.6 kD.
Distribution

Tissue Distribution: Cerebral cortex neurons in layers II-VI, and pyramidal neurons.
Cellular distribution: Golgi apparatus, mitochondria, nucleus, and cytoplasm in neuronal cell body and dendrites.

Function
Neurogranin is a neuron-specific, calmodulin-binding protein that participates in the protein kinase C signaling pathway. Neurogranin is a post-synaptic protein that modulates Ca2+ homeostasis in neurons by binding to and regulating the availability of calmodulin in the absence of Ca2+. Neurogranin function has been implicated in learning and memory.
Ligand Receptor
Calmodulin.
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References

1. Diez-Guerra FJ. 2010. IUBMB Life. 62(8): 597. (PubMed)
2. De Vos A, et al. 2016. J Alzheimers Dis. 53(4): 1523. (PubMed)
3. De Vos A. et al. 2015. Alzheimers Dement. 11(12):1461. (PubMed)

Gene ID
4900 View all products for this Gene ID

Related FAQs

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Go To Top Version: 1    Revision Date: 01/11/2017

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