Purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated Antibody

Pricing & Availability
Clone
SMI 33 (See other available formats)
Other Names
Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, neurofilament triplet H protein
Isotype
Mouse IgM, κ
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SMI33_PURE_Neurofilament_NonPhospho_Antibody_091517
IHC staining of purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated antibody (clone SMI 33) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using BioLegend’s Retrieve-ALL 3 pH 4.8 the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Kit was used for detection, followed by counterstaining with hematoxylin.
  • SMI33_PURE_Neurofilament_NonPhospho_Antibody_091517
    IHC staining of purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated antibody (clone SMI 33) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using BioLegend’s Retrieve-ALL 3 pH 4.8 the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Kit was used for detection, followed by counterstaining with hematoxylin.
  • SMI33_PURE_Neurofilament_NonPhospho_Antibody_2_051118
    Western blot of purified anti-Neurofilament H & M (NF-H/NF-M), Non-Phosphorylated antibody (clone SMI 33). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.25 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
835403 25 µg $95
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835404 100 µg $235
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Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton, and function primarily to provide structural support for the axon and to regulate the axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest NF (NF-L) runs at 68-70 kD. The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from the glia (negative for NF).

Product Details
Technical data sheet

Product Details

Reactivity
Rat, Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Immunohistochemistry. For Immunohistochemistry of paraffin-embedded tissues, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Cell bodies and dendrites are generally unstained. Other cells and tissues are unreactive except for peripheral axons.

Application References

(PubMed link indicates BioLegend citation)
  1. Wang J, et al. 2001. FEBS Lett. 19;507(1): 81-7. (WB)
RRID
AB_2716096 (BioLegend Cat. No. 835403)
AB_2716099 (BioLegend Cat. No. 835404)

Antigen Details

Structure
The medium or middle NF (NF-M) runs at about 145-160 kD, and the heavy or highest NF (NF-H) runs at 200-220 kD.
Distribution

Tissue Distribution: CNS, peripheral nerves and glandular cells of the prostate
Cellular distribution: cytoskeleton, nucleus, cytosol, and mitochondrion

Function
Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments, Phospho-Proteins
Gene ID
4744 View all products for this Gene ID
UniProt
View information about Neurofilament H & M (NF-H/NF-M) on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 09/20/2017

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