Purified anti-Myosin Heavy Chain Antibody

Pricing & Availability
Clone
Poly6212 (See other available formats)
Other Names
Myosin heavy chain, muscle
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly6212_022811
Rat carotid artery frozen sections were stained with Poly6212, followed by anti-rabbit Alexa Fluor® 594 and DAPI staining.
  • Poly6212_022811
    Rat carotid artery frozen sections were stained with Poly6212, followed by anti-rabbit Alexa Fluor® 594 and DAPI staining.
  • poly6212_110207
    Hela cells stained with purified rabbit polyclonal antibody against Myosin heavy chain (poly6212), followed by Cy3 conjugated Donkey anti-rabbit IgG and DAPI.
Cat # Size Price Quantity Avail. Save
621201 25 µl $85
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621202 100 µl $185
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Description

Myosin heavy chain is an actin-based motor protein with an approximate molecular weight of 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment). This protein is highly expressed in muscle. Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion. Myosin heavy chain is modified by acetylation in cysteine residues in the S1 domain for myosin ATPase activity. Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits that forms filaments.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Rat; reacts against 1960 aa length myosin heavy chain
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

IF - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

RRID
AB_2147412 (BioLegend Cat. No. 621201)
AB_2250964 (BioLegend Cat. No. 621202)

Antigen Details

Structure
Actin-based motor protein, approximately 223 kD. Myosin heavy chain can be split into 1 light meromysin and 1 heavy meromysin chain (and further into 2 globular subfragments and 1 rod-shaped subfragment)
Distribution
Highly expressed in muscle
Function
Motor proteins that convert chemical energy derived from ATP hydrolysis into mechanical force that drives motile processes such as cytokinesis, vesicular transport, and cellular locomotion
Interaction
Muscle myosin is a hexameric protein consisting of 2 heavy chain subunits and 2 alkali light chain subunits and 2 regulatory light chain subunits. Forms filaments
Modification
Alkylation
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Weiss A, et al. 1999. P. Natl. Acad. Sci. USA 96:2598.
2. Schiaffino S, et al. 1996. Physiol. Rev. 76:371 (review)

Regulation
Cysteine residues in the S1 domain are alkylated for myosin ATPase activity
Gene ID
4627 View all products for this Gene ID
UniProt
View information about Myosin heavy chain on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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