Purified anti-Myelin Basic Protein Antibody (Previously Covance catalog# SMI-99P)

Pricing & Availability
Clone
SMI 99 (See other available formats)
Other Names
MBP, Golli-MBP, myelin A1 protein, myelin membrane encephalitogenic protein
Previously
Covance Catalog# SMI-99P
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A_SMI99_PURE_Myelin_IHCP_HuBrain20x_100418
IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20X objective. Scale Bar: 50 µm
  • A_SMI99_PURE_Myelin_IHCP_HuBrain20x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20X objective. Scale Bar: 50 µm
  • B_SMI99_PURE_Myelin_IHCP_HuBrain10x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • C_SMI99_PURE_Myelin_IHCP_MuBrain10x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 50 µm
  • D_SMI99_PURE_Myelin_IHCP_MuBrain40x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • E_SMI99_PURE_Myelin_IHCP_RatBrain40x_100418
    IHC staining of purified anti-Myelin Basic Protein antibody (clone SMI 99) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 100X (Cat. No. 927601), the tissue was incubated with 0.25 µg/ml of the primary antibody overnight at 4°C, followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
  • F_SMI99_PURE_Myelin_WB_10ugload_100418
    Western blot of purified anti-Myelin Basic Protein antibody (clone SMI 99). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
808403 25 µl $95
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808401 100 µl $235
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808402 500 µl $425
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Description

Myelin basic protein (MBP) is a protein involved in the myelination of nerves in the CNS. MBP functions to maintain the correct structure of myelin, through its interaction with the lipids in the myelin membrane. The myelin sheath is made up of MBP and lipids, and acts as an insulator to increase the velocity of axonal impulse conduction. MBP plays a central role in the demyelinating disease multiple sclerosis (MS). A hallmark of the disease is the loss of the myelin sheath surrounding nerves, thought to be induced by antibodies against MBP.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution + 0.1% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Validated
ICC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.25 – 0.5 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.2 – 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody reacts with the sequence Ala-Ser-Asp-Tyr-Lys-Ser in position 131-136 of the classic human myelin basic protein. The antibody detects myelin basic protein from most mammalian species. This antibody detects developing and adult myelin, developing oligodedrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain sections. The combination of this antibody with clone SMI-91 and/or SMI-94 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Application References

(PubMed link indicates BioLegend citation)
  1. Fyffe-Maricich SL, et al. 2011. J. Neurosci. 31(3):843-50. (IHC, WB) PubMed
  2. Mendz GL, et al. 1985. Biochem. 228(1):61-8.
  3. Kerlero De Rosbo N, et al. 1984. Neurochem. Res. 9(10):1359-69.
  4. Carnegie PR, et al. 1983. J. Neuroimmunol. 5(2):125-34.
  5. Dowse CA, et al. 1983. J. Neuroimmunol. 5(2):135-44.
  6. Del Valle L,et al. Cancer Biol Ther. 9:286. (IHC) PubMed
  7. Kiryu-Seo S, et al. 2010. J. Neurosci. 30(19):6658-66. (ICC) PubMed
  8. Samanta J, et al. 2010. Stroke. 41(2):357-62. (IHC) PubMed
  9. Werner HB, et al. 2007. J. Neurosci. 27(29):7717-30. (WB, ICC) PubMed
Product Citations
  1. Fyffe-Maricich S, et al. 2011. J Neurosci. 31:843-850. PubMed
  2. Valle L, et al. 2010. Cancer Biol Ther. 9:286-294. PubMed
  3. Kiryu-Seo S, et al. 2010. J Neurosci. 30:6658-6666. PubMed
  4. Samanta J, et al. 2010. Stroke. 41:357-362. PubMed
  5. Werner H, et al. 2007. J Neurosci. 27:7717-7730. PubMed
  6. Liu J, et al. 2016. J Neurosci. 36: 957 - 962. PubMed
  7. Hernandez M, et al. 2016. J Neurosci. 36: 806 - 813. PubMed
  8. Luo F, et al. 2016. J Neurosci. 36: 3024 - 3037. PubMed
  9. Battefeld A, Klooster J, Kole M 2016. Nat Commun. 7:11298. PubMed
  10. Safaiyan S, et al. 2016. Nat Neurosci. 10.1038/nn.4325. PubMed
  11. Shi Y, et al. 2016. Mol Pain. 10.1177/1744806916656845. PubMed
  12. Jeffries M, et al. 2016. J Neurosci. 36: 9186 - 9200. PubMed
  13. Barron T 2018. Scientific reports. 8:1022. PubMed
  14. Yermakov LM 2018. Frontiers in cellular neuroscience. 12:146. PubMed
  15. Larson VA 2018. eLife. 7: e34829. PubMed
Publication Library
RRID
AB_2734562 (BioLegend Cat. No. 808403)
AB_2564741 (BioLegend Cat. No. 808401)
AB_2564742 (BioLegend Cat. No. 808402)

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Go To Top Version: 4    Revision Date: 10/04/2018

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