Purified anti-MyD88 Antibody

Pricing & Availability
Clone
O91B8 (See other available formats)
Other Names
Myeloid Differentiation Primary Response Protein MyD88, MYD88D
Isotype
Mouse IgG1
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Product Citations
publications
O91B8_Purified_MyD88_Antibody_WB_081516
Total cell lysate from 293E cells (lane 1, 15 µ), Jurkat cells (lane 2, 15 µg) and Raw264.7 (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-MyD88 antibody (clone O91B8). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • O91B8_Purified_MyD88_Antibody_WB_081516
    Total cell lysate from 293E cells (lane 1, 15 µ), Jurkat cells (lane 2, 15 µg) and Raw264.7 (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-MyD88 antibody (clone O91B8). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
Cat # Size Price Quantity Avail. Save
675802 100 µg $195
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Description

MyD88, originally isolated as myeloid differentiation primary response gene, is expressed in a variety of tissues.  MyD88 serves as a cytoplasmic adaptor protein involved in the signaling of TLR and IL-1R family members.  It associates with and recruits IRAK to IL-1R complex in response to IL-1. This pathway leads to activation of NF-kB. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18. MyD88 deficient mice lack a response to LPS (a bacterial product) which employs toll like receptors 2 and 4 (TLR2 and TLR4) as signaling receptors.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length recombinant MyD88 protein produced in 293T cells.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Affinity purified.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
RRID
AB_2565607 (BioLegend Cat. No. 675802)

Antigen Details

Structure
A 296 amino acid protein encoded by 5 exons. MW around 33 kD.
Distribution
Expressed by various tissues including lymphocytes, monocytes, heart, liver and kidney.
Function
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response.
Interaction
TIRAP, TLR2, TLR4, IRAK1, IRAK2, IL18R1, IRF-1 et al.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Bonnert TP, et al. 1997. FEBS Letters 402:81.
2. Burns K, et al. 1998. J. Biol. Chem. 20:12203.
3. Ohnishi H, et al. 2009. Proc. Natl. Acad. Sci. USA 106:10260.

Gene ID
4615 View all products for this Gene ID
UniProt
View information about MyD88 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08/15/2016

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