- RME-1/CD62E (See other available formats)
- Other Names
- E-selectin, ELAM-1, LECAM-1
- Mouse IgG1, κ
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- Product Citations
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CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I transmembrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells. CD62E is involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Sialyl Lewis X (CD15s) and PSGL-1 (CD162).Product Details
- Mouse, Rat
- Antibody Type
- Host Species
- Rat E-selectin transfected chinese hamster ovary cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
IF - Quality tested
IHC-F - Validated
WB, IP, ELISA, Blocking - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 5-10 μg/ml is recommended. For immunohistochemistry, a concentration range of 2.5-5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5, Western blotting1, immunoprecipitation1, ELISA7, and blocking1,2,3. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 148803) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01EU/µg).
(PubMed link indicates BioLegend citation)
- Walter UM, et al. 1997. Hybridoma 16:249. (Block, WB, IP)
- Walter UM, et al. 1997. Immunology 92:290. (Block, Rat Reactivity)
- Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block, Mouse Reactivity)
- Bernardes-Silva M, et al. 2001. J. Cereb. Blood Flow Metab. 21:1115. (IHC)
- Alvarez A, et al. 2004. Blood 104:402. (IHC, Rat Reactivity)
- Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
- Walter UM, et al. 1997. in Hybridoma 16:355. (ELISA)
- Product Citations
AB_2564222 (BioLegend Cat. No. 148801)
AB_2564223 (BioLegend Cat. No. 148802)
- Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Highly expressed on activated endothelial cells
- Involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
- Ligand Receptor
- Sialyl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported to interact with CD62E
- Cell Type
- Endothelial cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Adhesion Molecules
- Antigen References
1. Collins T, et al. 1991. J. Biol. Chem. 266:2466
2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238
3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
4. Lawrence MB, et al. 1993. J. Immunol. 151:6338.
5. Walz G, et al. 1990. Science 250:1132
- Gene ID
- 20351 View all products for this Gene ID
- View information about CD62E on UniProt.org
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