Purified anti-mouse/rat CD62E (E-selectin) Antibody

Pricing & Availability
Clone
RME-1/CD62E (See other available formats)
Other Names
E-selectin, ELAM-1, LECAM-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
RME-1_Purified_CD62E_Antibody_1_IF_092414
Mouse brain endothelial cell line bEND.3 cells were stimulated with TNF-α overnight. Then the cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes, subsequently stained with 5 µg/ml of purified anti-mouse/rat CD62E (clone RME-1/CD62E) (red) in blocking buffer for 3 hours at room temperature followed by DyLight™ 594 Goat anti-mouse IgG for another 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • RME-1_Purified_CD62E_Antibody_1_IF_092414
    Mouse brain endothelial cell line bEND.3 cells were stimulated with TNF-α overnight. Then the cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes and blocked with 5% FBS for 30 minutes, subsequently stained with 5 µg/ml of purified anti-mouse/rat CD62E (clone RME-1/CD62E) (red) in blocking buffer for 3 hours at room temperature followed by DyLight™ 594 Goat anti-mouse IgG for another 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • RME-1_Purified_CD62E_Antibody_2_IHC-F_102416
    C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of purified anti-mouse CD62E (clone RME-1/CD62E) overnight at 4°C followed by 2.5µg of anti-mouse IgG Alexa Fluor® 488 (red) and Alexa Fluor® 594 anti-mouse CD326/Ep-CAM (clone G8.8) antibody (green) for 2 hours at room temper
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Cat # Size Price Quantity Avail. Save
148801 50 µg $75
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148802 500 µg $275
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Description

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I transmembrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells. CD62E is involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Sialyl Lewis X (CD15s) and PSGL-1 (CD162).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat E-selectin transfected chinese hamster ovary cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IF - Quality tested
IHC-F - Validated
WB, IP, ELISA, Blocking - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 5-10 μg/ml is recommended. For immunohistochemistry, a concentration range of 2.5-5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5, Western blotting1, immunoprecipitation1, ELISA7, and blocking1,2,3. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 148803) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Walter UM, et al. 1997. Hybridoma 16:249. (Block, WB, IP)
  2. Walter UM, et al. 1997. Immunology 92:290. (Block, Rat Reactivity)
  3. Hickey MJ, et al. 1998. Circ. Res. 83:1124. (Block, Mouse Reactivity)
  4. Bernardes-Silva M, et al. 2001. J. Cereb. Blood Flow Metab. 21:1115. (IHC)
  5. Alvarez A, et al. 2004. Blood 104:402. (IHC, Rat Reactivity)
  6. Piccio L, et al. 2002. J. Immunol. 168:1940. (IF)
  7. Walter UM, et al. 1997. in Hybridoma 16:355. (ELISA)
RRID
AB_2564222 (BioLegend Cat. No. 148801)
AB_2564223 (BioLegend Cat. No. 148802)

Antigen Details

Structure
Member of the selectin family, 115 kD type I membrane protein; contains one C-type lectin domain and one EGF-like domain
Distribution

Highly expressed on activated endothelial cells

Function
Involved in leukocyte tethering and rolling on activated endothelium at inflammatory sites, may also play a role in tumor metastasis and angiogenesis
Ligand Receptor
Sialyl Lewis X (CD15s) and PSGL-1 (CD162) bind to CD62E. Recently, CLA (cutaneous lymphocyte antigen) has also been reported to interact with CD62E
Cell Type
Endothelial cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Adhesion Molecules
Antigen References

1. Collins T, et al. 1991. J. Biol. Chem. 266:2466
2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238
3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
4. Lawrence MB, et al. 1993. J. Immunol. 151:6338.
5. Walz G, et al. 1990. Science 250:1132

Gene ID
20351 View all products for this Gene ID
UniProt
View information about CD62E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10/24/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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