- HMβ1-1 (See other available formats)
- Other Names
- integrin β1, VLA-β chain, β1 integrin, GPIIa, ITGB1
- Armenian Hamster IgG
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- Product Citations
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CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMß1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.Product Details
- Mouse, Rat
- Antibody Type
- Host Species
- Armenian Hamster
- Purified mouse VLA-4 (α4β1, CD49d/CD29)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-F - Validated
IP, Block - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry4 of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses1, and in vivo inhibition of neutrophil migration2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102210).
(PubMed link indicates BioLegend citation)
- Noto K, et al. 1995. Int. Immunol. 7:835.
- Ridger VC, et al. 2001. J. Immunol. 166:3484.
- Jia W, et al. 2005. Blood 106:3854.PubMed
- Economopoulou M, et al. 2005. Blood 106:3831.
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed
- Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
- Kim DT, et al. 2008. Blood 111:2929.PubMed
- Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
- Carlson TR, et al. 2008. Development. 135:2193. PubMed
- Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed
- Baker CM, et al. 2012. PNAS. PubMed.
- Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
- Product Citations
AB_312878 (BioLegend Cat. No. 102201)
AB_312879 (BioLegend Cat. No. 102202)
- Integrin family, 130 kD
Leukocytes, endothelial cells, smooth muscle, epithelial cells
- Extracellular matrix
- Cell Type
- Embryonic Stem Cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal Stem Cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Springer TA. 1990. Nature 346:425.
- Gene ID
- 16412 View all products for this Gene ID 24511 View all products for this Gene ID
- View information about CD29 on UniProt.org
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