Purified anti-mouse Panendothelial Cell Antigen Antibody

Pricing & Availability
MECA-32 (See other available formats)
Other Names
Pan-endothelial Cell Antigen, MECA-32, Plasmalemma vesicle-associated protein
Rat IgG2a, κ
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Product Citations
bEND.3 cells stained with MECA-32 Alexa Fluor® 488
  • MECA-32_A488_061109.jpg
    bEND.3 cells stained with MECA-32 Alexa Fluor® 488
Cat # Size Price Quantity Avail. Save
120501 50 µg $60
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120502 500 µg $220
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MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic and the adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But in some conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and the neurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to day 16 of gestation, and disappears at day 17 and after.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Mouse lymph node stromal cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
ICC, IHC-F, IP, WB - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (in the relevant formats) include: immunoprecipitation, Western blotting, immunocytochemistry, and immunohistochemical staining of acetone-fixed frozen sections2-5. Clone MECA-32 is not recommended for formalin-fixed paraffin sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Leppink DM, et al. 1989. Transplantation 48:874.
  2. Bergese SD, et al. 1994. Transplantation 57:711. (IHC)
  3. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581. (IHC)
  4. Kopp HG, et al. 2005. Blood 106:505. (IHC)
  5. Hallmann R, et al. 1995. Dev. Dyn. 202:325. (IHC)
Product Citations
  1. Shono Y, et al. 2010. Blood. 115:5401. PubMed
  2. Chee J, et al. 2011. J Immunol. 187:1702. PubMed
  3. Zheng J, et al. 2011. Blood. 117:470. PubMed
  4. Lopez-Dee Z, et al. 2015. PLoS One. 10: e0139918. PubMed
  5. Qiao J, et al. 2015. Sci Rep. 5: 17828. PubMed
  6. Alfaro D, et al. 2015. J Leukoc Biol. 98: 883 - 896. PubMed
Publication Library
AB_493301 (BioLegend Cat. No. 120501)
AB_493302 (BioLegend Cat. No. 120502)

Antigen Details

50-55 kD homodimer

Endothelial cell in the embryonic and the adult mice, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution

Cell Type
Embryonic Stem Cells, Endothelial cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules
Antigen References

1. Leppnik DM, et al. 1989. Transplantation 48:874.
2. Hallmann R, et al. 1995. Dev. Dyn. 202:325.
3. Kruse A, et al. 1999. Bio. Reprod. 61:1393.

Gene ID
84094 View all products for this Gene ID
View information about Panendothelial Cell Antigen on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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