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Purified anti-mouse Panendothelial Cell Antigen Antibody

Pricing & Availability
Clone
MECA-32 (See other available formats)
Other Names
Pan-endothelial Cell Antigen, MECA-32, Plasmalemma vesicle-associated protein
Isotype
Rat IgG2a, κ
Ave. Rating
0 reviews
MECA-32_A488_061109.jpg
bEND.3 cells stained with MECA-32 Alexa Fluor® 488
  • MECA-32_A488_061109.jpg
    bEND.3 cells stained with MECA-32 Alexa Fluor® 488
Cat # Size Price Quantity Avail. Save
120501 50 µg $60.00
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120502 500 µg $220.00
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Description

MECA-32 is a 50-55 kD homodimer glycoprotein primarily expressed on most endothelial cells in the embryonic and the adult mouse tissues, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution. In skeletal and cardiac muscle, the MECA-32 antigen is confined in small arterioles and venules. But in some conditions, such as inflammation or cardiac transplantation, MECA-32 expression can be induced on some negative-cardiac endothelial cells. In the brain, its expression occurs only in the circumventricular organs and the neurohemal tissues. During embryonic development, MECA-32 antigen is expressed on the brain vasculature up to day 16 of gestation, and disappears at day 17 and after.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Immunogen
Mouse lymph node stromal cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB, ICC, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (in the relevant formats) include: immunoprecipitation, Western blotting, immunocytochemistry, and immunohistochemical staining of acetone-fixed frozen sections2-5. Clone MECA-32 is not recommended for formalin-fixed paraffin sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Leppink DM, et al. 1989. Transplantation 48:874.
  2. Bergese SD, et al. 1994. Transplantation 57:711. (IHC)
  3. Tanneau GM, et al. 1999. J. Histochem. Cytochem. 47:1581. (IHC)
  4. Kopp HG, et al. 2005. Blood 106:505. (IHC)
  5. Hallmann R, et al. 1995. Dev. Dyn. 202:325. (IHC)
  6. Shono Y, et al. 2010. Blood. 115:5401. PubMed
  7. Chee J, et al. 2011. J Immunol. 187:1702. PubMed
  8. Zheng J, et al. 2011. Blood. 117:470. PubMed
  9. Lopez-Dee Z, et al. 2015. PLoS One. 10: e0139918. PubMed
  10. Qiao J, et al. 2015. Sci Rep. 5: 17828. PubMed
  11. Alfaro D, et al. 2015. J Leukoc Biol. 98: 883 - 896. PubMed
Publication Library
RRID
AB_493301 (BioLegend Cat. No. 120501)
AB_493302 (BioLegend Cat. No. 120502)

Antigen Details

Structure
50-55 kD homodimer
Distribution
Endothelial cell in the embryonic and the adult mice, except in the brain and in skeletal and cardiac muscle, where it has more restricted distribution
Antigen References

1. Leppnik DM, et al. 1989. Transplantation 48:874.
2. Hallmann R, et al. 1995. Dev. Dyn. 202:325.
3. Kruse A, et al. 1999. Bio. Reprod. 61:1393.

Gene ID
84094 View all products for this Gene ID
UniProt
View information about Panendothelial Cell Antigen on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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