Purified anti-mouse Ki-67 Recombinant Antibody

Pricing & Availability
Clone
QA18A32 (See other available formats)
Regulatory Status
RUO
Other Names
Mki67, Ki67, Ki-67, MIB-1, KIA
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
1_QA18A32_PURE_Ki-67_RECOM_IHC_102221
C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse Ki-67 (clone QA18A32) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 1_QA18A32_PURE_Ki-67_RECOM_IHC_102221
    C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse Ki-67 (clone QA18A32) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 2_QA18A32_PURE_Ki-67_RECOM_ICC_102221
    TE‐71 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X‐100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 μg/mL of purified anti-mouse Ki-67 (clone QA18A32) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
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163202 100 µg $127
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Immunogen
E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2904309 (BioLegend Cat. No. 163202)

Antigen Details

Structure
325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Distribution

Nucleus and chromosomes

Function
Required for cell cycle progression and proliferation.
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Antigen References
  1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
  2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
  3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
  4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
  5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
  6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID
4288 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/22/2021

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