Purified anti-mouse IL-3 Antibody

Pricing & Availability
Clone
MP2-8F8 (See other available formats)
Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
Isotype
Rat IgG1, κ
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Product Citations
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Cat # Size Price Quantity Avail. Save
503901 50 µg $90
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503902 500 µg $240
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Description IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The MP2-8F8 antibody can neutralize the bioactivity of natural or recombinant IL-3. Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed, recombinant mouse IL-3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
IP, IHC - Reported in the literature

Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-3 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
ELISA or ELISPOT Capture1-5,8: The purified MP2-8F8 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP2-43D11 antibody (Cat. No. 504002) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  2. Abrams, J., et al. 1988. J. Immunol. 140:131.
  3. Cockayne, D., et al. 1991. Growth Factors 5:171.
  4. Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
  5. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  6. Finkelman, F., et al. 1993. J. Immunol. 151:1235.
  7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Karulin, A., et al. 2000. J. Immunol. 164:1862.
RRID
AB_315307 (BioLegend Cat. No. 503901)
AB_315308 (BioLegend Cat. No. 503902)

Antigen Details

Structure
Cytokine; 28 kD (Mammalian)
Bioactivity
Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
Cell Sources
Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
Cell Targets
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors
Heterodimer IL-3Rα (CD123) and two alternate β-subunits, AIC2A binds IL-3 and is specific to IL-3Rα, AIC2B (CDw131) shared in common with IL-5R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Frendl, G., 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle, J., 1992. Chem. Immunology 51:65.
4. Valen T, P., et al. 1990. Blut 61:338.
Regulation
Upregulated by T cell stimulation; downregulated by glucocorticoids
Gene ID
16187 View all products for this Gene ID
UniProt
View information about IL-3 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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