Purified anti-mouse IL-17A Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E. coli expressed, recombinant mouse IL-17A

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
CyTOF® - Verified
ELISPOT Capture, ICFC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-17 recombinant protein ranging from 1000 to 15.63 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture^3,4 and ELISPOT Capture⁵: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry^{2-4,7,8,11,12}: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization^6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References

(PubMed link indicates BioLegend citation)

1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed

Product Citations

1. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
2. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
3. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
4. Yeola AP, et al. 2020. Front Immunol. 10:3115. PubMed
5. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
6. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
7. Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
8. Guo R, et al. 2020. Cell Res. 30:21. PubMed
9. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
10. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
11. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
12. Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
13. He Z, et al. 2017. J Immunol. 199:955. PubMed
14. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
15. Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
16. Volchenkov R, et al. 2017. Front Immunol. 0.818055556. PubMed
17. Cooney L, et al. 2011. J Immunol. 187:4440. PubMed
18. Mencarelli A, et al. 2018. Front Immunol. 9:261. PubMed
19. Kaisar MMM, et al. 2018. PLoS Biol. 16:e2005504. PubMed
20. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
21. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
22. Hua J, et al. 2018. Sci Rep. 5.235416667. PubMed
23. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
24. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
25. Chen Y, et al. 2017. J Autoimmun. 77:96. PubMed
26. Cardoso F, et al. 2021. Nature. 597:410. PubMed
27. Yoon S, et al. 2020. Antioxidants (Basel). 10:. PubMed
28. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
29. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
30. Oyler‐Yaniv J et al. 2017. Molecular cell. 66(5):635-647 . PubMed
31. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
32. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
33. Takeuchi Y, et al. 2021. Sci Rep. 11:18679. PubMed
34. Wang Y, et al. 2021. Front Immunol. 11:601601. PubMed
35. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
36. Nguyen H, et al. 2020. Clin Cancer Res. 26:3481. PubMed
37. Smith E, et al. 2008. J Immunol. 181:1357. PubMed
38. Jordan J, et al. 2008. Infect Immun. 76:3717. PubMed
39. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed

RRID

AB_315461 (BioLegend Cat. No. 506901)
AB_315462 (BioLegend Cat. No. 506902)

Antigen Details

Structure

Cytokine; dimer; 15 kD (Mammalian).

Bioactivity

Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells

Cell Sources

CD4⁺ memory T cells

Cell Targets

Fibroblasts, epithelial and endothelial cells, stromal cells

Receptors

IL-17R (CD217)

Biology Area

Cell Biology, Immunology, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID

16171 View all products for this Gene ID

UniProt

View information about IL-17A on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Active Protocols: Sandwich ELISA - Video
• Sandwich ELISA Protocol

Related Pages & Pathways

Related FAQs

Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?

Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.

Other Formats

Certificate of Analysis