Purified anti-mouse GITR Ligand Antibody

Pricing & Availability
5F1 (See other available formats)
Regulatory Status
Other Names
Rat IgG2a, κ
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GITR ligand transfected cells were stained with purified GITR ligand (clone 5F1, filled histogram) or purified rat IgG2a, κ isotope control (open histogram), followed by anti-rat IgG PE.
  • 5F1_Purified_GITRL_Antibody_FC_042414
    GITR ligand transfected cells were stained with purified GITR ligand (clone 5F1, filled histogram) or purified rat IgG2a, κ isotope control (open histogram), followed by anti-rat IgG PE.
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147402 100 µg $248
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GITR ligand (GITRL) is a member of the TNF superfamily, also known as TNFSF18 or TL6. It is expressed on B cells, macrophages, dendritic cells, and endothelial cells. The expression of GITRL on the dendritic cell surface can be transiently upregulated, and then rapidly downregulated, by LPS stimulation. GITRL binds to its receptor, GITR, to costimulate T cell activation, proliferation, and cytokine production, inhibit the suppressive functions of CD4+CD25+ Tregs, and induce macrophage activation. GITR-GITRL interaction has been implicated as an essential regulator of the immune response to self antigens and phathogens.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additonal reported applications (for relevant formats of thes clone) include: inhibition of GITR-GITRL mediated T cell proliferation and reversing Treg-mediated suppression1.

The following patents apply to clone 5F1: U.S. Patent Serial No. 7,618,632 and Japan Patent Serial No. 4638876.

Application References

(PubMed link indicates BioLegend citation)
  1. Stephens GL, et al. 2004. J. Immunol. 173:5008. (Block)
AB_2563295 (BioLegend Cat. No. 147402)

Antigen Details

TNF superfamily member
B cells, macrophages, dendritic cells, endothelial cells
Binds GITR receptor to costimulate T cell activation, proliferation, and cytokine production; inhibits the suppressive activities of CD4+CD25+ Tregs
Cell Type
B cells, Macrophages, Dendritic cells, Endothelial cells, Tregs
Biology Area
Antigen References

1. Tone M, et al. 2003. Proc. Natl. Acad. Sci. USA 100:15059.
2. Kim JD, et al. 2003. Genes Immun. 4:564.
3. Yu KY, et al. 2003. Biochem. Biophys. Res. Commun. 310:433.
4. Shin HH, et al. 2002. Cytokine 19:187.

Gene ID
240873 View all products for this Gene ID
View information about GITR Ligand on UniProt.org

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Go To Top Version: 2    Revision Date: 05/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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