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Purified anti-mouse CD68 Antibody

Pricing & Availability
Clone
FA-11 (See other available formats)
Other Names
Macrosialin
Isotype
Rat IgG2a
Ave. Rating
1 reviews
FA-11_A647_021810
Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 Alexa Fluor® 647
  • FA-11_A647_021810
    Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 Alexa Fluor® 647
Cat # Size Price Quantity Avail. Save
137001 50 µg $85.00
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137002 500 µg $365.00
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Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Immunogen
Purified Con A receptor glycoproteins from the P815 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
FC - Validated
IP, WB, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, and immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3.

Application References

(PubMed link indicates BioLegend citation)
  1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
  2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
  3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
  4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
  5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
  8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  9. Kayama H, et al. 2012. Proc Natl Acad Sci U S A. 109:5010. PubMed
  10. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  11. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  12. McKinstry S, et al. 2014. J Neurosci. 34:9455. PubMed
  13. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  14. Mori H, et al. 2015. Toxicol Pathol. 43: 883-889. PubMed
  15. Gomez-Pastor R, et al. 2017. Nat Commun. 8:14405. PubMed
  16. Phua T, et al. 2017. Sci Rep. 10.1038/srep44351. PubMed
  17. Milasan A, et al. 2017. J Am Heart Assoc. 10.1161/JAHA.117.006892. PubMed
  18. Yi L, et al. 2017. Sci Rep. 7:7953. PubMed
Publication Library
RRID
AB_2044003 (BioLegend Cat. No. 137001)
AB_2044004 (BioLegend Cat. No. 137002)

Antigen Details

Structure
A member of the lysosomal-associated membrane protein (lamp) family.
Distribution
Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function
Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Antigen References

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.

Gene ID
12514 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/26/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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