Purified anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
2 reviews
Product Citations
publications
30-F11_Pure_071608.jpg
BALB/c splenocytes stained with 30-F11 purified, followed by anti-rat IgG FITC
  • 30-F11_Pure_071608.jpg
    BALB/c splenocytes stained with 30-F11 purified, followed by anti-rat IgG FITC
  • 30-F11_Pure_CD45_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified CD45 clone 30-F11 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with CollagenIV Cy5 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # Size Price Quantity Avail. Save
103101 50 µg $30
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103102 500 µg $95
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, CyTOF® - Validated
IP, CMCD, IHC, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,  immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103120).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Podd B, et al. 2006. J Immunol. 176:6532. PubMed
  2. Latasa M, et al. 2010. PLoS One. 5:e15690. PubMed
  3. DeFalco T, et al. 2014. Proc Natl Acad Sci U S A. 111:2384. PubMed
  4. Nikolaou K, et al. 2014. EMBO J. 34:430. PubMed
  5. Cha J, et al. 2015. J Biol Chem. 290:15337. PubMed
  6. Gereke M, et al. 2012. J Vis Exp. 70: 4322. PubMed
  7. Davies M, et al. 2017. PLoS Pathogens. 13(6):e1006435. PubMed
  8. Cédile O, Wlodarczyk A, and Owens T 2017. APMIS.. 10.1111/apm.12740. PubMed
  9. Ramos J, et al. 2017. Front Immunol.. 10.3389/fimmu.2017.01666. PubMed
  10. Sutton C, et al. 2017. Nat Commun.. 10.1038/s41467-017-02111-0. PubMed
  11. Seehus C, et al. 2017. Nat Commun.. 10.1038/s41467-017-02023-z. PubMed
  12. Rhee S, et al. 2018. Nat Commun. 9:368. PubMed
  13. Mrdjen D 2018. Immunity. 48:380. PubMed
  14. Jones KB 2018. Cell stem cell. 24:183. PubMed
  15. Murai K 2018. Cell stem cell. 23:687. PubMed
Publication Library
RRID
AB_312966 (BioLegend Cat. No. 103101)
AB_312967 (BioLegend Cat. No. 103102)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
Mesenchymal Stem Cells, B cells, Dendritic cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10/25/2018

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