Purified anti-mouse CD366 (Tim-3) Recombinant Antibody

Pricing & Availability
QA20A20 (See other available formats)
Regulatory Status
Other Names
CD366, Tim-3, Hepatitis A virus cellular receptor 2 homolog (HAVcr-2)
Mouse IgG1, κ
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Product Citations
Mouse Tim-3 transfected cells stained with purified anti-mouse CD366 (Tim-3) recombinant (clone QA20A20) (filled histogram), or purified mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
  • QA20A20_PURE_CD366_Antibody_061022
    Mouse Tim-3 transfected cells stained with purified anti-mouse CD366 (Tim-3) recombinant (clone QA20A20) (filled histogram), or purified mouse IgG1, κ isotype control (open histogram) followed by PE anti-mouse IgG.
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165402 100 µg $286
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CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
The antibody was purified by affinity chromatography.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA20A20 completely blocks the binding of reference clone RMT3-23 on target cells.

AB_2924497 (BioLegend Cat. No. 165402)

Antigen Details

Transmembrane protein containing immunoglobulin domain and mucin-like domain with a predicted molecular weight of 31 kD; can exist as a soluble form lacking mucin and transmembrane domains.

Expressed on activated Th1 lymphocytes and CD11b+ macrophages

May play a role in the development of immune responses and the development of Th1-mediated responses
Macrophages, Th1
Putative ligand on resting CD4+ lymphocytes
Cell Type
Dendritic cells, Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Sabatos CA, et al. 2003. Nat Immunol. 4:1102-10.
  2. Kuchroo VK, et al. 2003. Nat Rev Immunol. 3:454-62.
  3. Mooney L, et al. 2002. Nature. 415:536-41.
Gene ID
171285 View all products for this Gene ID
View information about CD366 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/10/2022

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  • Purified anti-mouse CD366 (Tim-3) Recombinant Antibody


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