Purified anti-mouse CD34 Antibody

Pricing & Availability
Clone
MEC14.7 (See other available formats)
Other Names
Mucosialin
Isotype
Rat IgG2a, κ
Ave. Rating
1 reviews
Product Citations
publications
MEC14-7_Pure_122607
Mouse NIH/3T3 cell line stained with purified MEC14.7, followed with biotinylated anti-rat IgG and Sav-PE
  • MEC14-7_Pure_122607
    Mouse NIH/3T3 cell line stained with purified MEC14.7, followed with biotinylated anti-rat IgG and Sav-PE
  • MEC14-7_Purified_CD34_Antibody_IF_012914
    NIH3T3 cells were fixed with 1% PFA and then stained with 10 µg/1ml purified CD34 (clone MEC14.7) for 3 hours at room temperature. The cells were then stained with 2.5 mg/ml anti-rat IgG Dylight™ 594 secondary antibody (red) for 1 hours at room temperature. The nuclei were counterstained with DAPI and are shown in blue. The image was captured by 40X objective.
  • MEC14dot7_PURE_CD34_Antibody_IHC-F_062118
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD34 (clone MEC14.7) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG (clone Poly4054, red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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Cat # Size Price Quantity Avail. Save
119301 50 µg $60
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119302 500 µg $250
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Description

CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Cells transfected with mouse CD34
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-F - Validated
WB, IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of acetone-fixed frozen sections and paraffin-embedded sections2,4,5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
  2. Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
  3. Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
  4. Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
  5. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  6. Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
  7. Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
  8. Zaynagetdinov R., et al. 2011. J Immunol. 187:5703. PubMed.
Product Citations
  1. Zaynagetdinov R, et al. 2011. J Immunol. 187:5703. PubMed
  2. Zhao J, et al. 2015. PLoS One. 10: 0131674. PubMed
  3. Uhlik M, et al. 2016. Cancer Res . 76: 2573 - 2586. PubMed
  4. Lin XJ, et al. 2018. Mol Ther Nucleic Acids. 9:1127. PubMed
  5. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
Publication Library
RRID
AB_345279 (BioLegend Cat. No. 119301)
AB_345280 (BioLegend Cat. No. 119302)

Antigen Details

Structure
Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported
Distribution

Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow

Function
Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins.
Ligand/Receptor
L-selectin, other selectins
Cell Type
Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368.
2. Brown J, et al. 1991. Int. Immunol. 3:175.
3. Suda J, et al. 1992. Blood 79:2288.
4. Baumhueter S, et al. 1994. Blood 84:2554.

Gene ID
12490 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/18/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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