Purified anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_Pure_060308
TE-71 cell line stained with G8.8 purified, followed by anti-rat IgG PE
  • G8dot8_Pure_060308
    TE-71 cell line stained with G8.8 purified, followed by anti-rat IgG PE
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118201 50 µg $71
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118202 500 µg $282
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Thorsen AS, et al. 2020. Dis Model Mech. 14:dmm046706. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  4. Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
  5. Nudel I, et al. 2011. J Immunol. 186:891. PubMed
  6. Huang L, et al. 2020. JCI Insight. 5:00. PubMed
  7. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  8. Casey AE, et al. 2018. J Cell Biol. 217:2951. PubMed
  9. Karabacak NM, et al. 2022. Cancer Res. 82:1084. PubMed
  10. Hirokawa Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 306:547. PubMed
  11. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  12. Ishii K, et al. 2012. Development. 139:1734. PubMed
  13. Aparicio-Domingo P, et al. 2015. J Exp Med. 101084/jm20150318. PubMed
  14. Foster DS, et al. 2020. Nat Commun. 3.278472222. PubMed
  15. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  16. Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
  17. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  18. Fuhrmann L, et al. 2021. Int J Mol Sci. 22:. PubMed
  19. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  20. E-Lacerda RR, et al. 2020. Clin Exp Allergy. 256:50. PubMed
  21. Corcos N, et al. 2021. Frontiers in Immunology. 12:616215. PubMed
  22. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  23. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  24. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  25. Billings SE, et al. 2021. Development. 148: . PubMed
  26. Tomchaney M, et al. 2021. BMC Pulm Med. 21:275. PubMed
  27. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  28. Thorsen AS, et al. 2020. Dis Model Mech. . PubMed
  29. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  30. Yamamoto K, et al. 2022. STAR Protoc. 3:101155. PubMed
  31. Yamamoto K, et al. 2021. iScience. 24:103064. PubMed
  32. Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
  33. Burger ML, et al. 2021. Cell. 184:4996. PubMed
  34. Molina LM, et al. 2021. Cell Reports. 36(1):109310. PubMed
  35. Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed
  36. Van Winkle JA, et al. 2020. J Virol. 94:. PubMed
  37. Iyer N, et al. 2020. PLoS Pathog. 16:e1008360. PubMed
  38. Zhou Y, et al. 2020. Am J Pathol. 190:1414. PubMed
  39. Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
RRID
AB_1089026 (BioLegend Cat. No. 118201)
AB_1089027 (BioLegend Cat. No. 118202)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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