- MEC13.3 (See other available formats)
- Other Names
- PECAM-1, EndoCAM
- Rat IgG2a, κ
- Ave. Rating
- 1 reviews
- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.Product Details
- Antibody Type
- Host Species
- Polyoma middle T transformed EC line tEnd.1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IP, Block, IHC-F - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102512).
(PubMed link indicates BioLegend citation)
- Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
- Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
- DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
- Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
- Baldwin HS, et al. 1994. Development 120:2539. (IHC)
- Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
- Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
- Chacko AM, et al. 2012. PLoS One 7:e34958.
- Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
- Morita R, et al. 2015. PNAS. 112:160. PubMed
- Ito A, et al. 2015. Brain Res. 1594:310. PubMed
- Product Citations
AB_312908 (BioLegend Cat. No. 102501)
AB_312909 (BioLegend Cat. No. 102502)
- Ig superfamily, 130-140 kD
Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
- CD38, αV/β3 integrin
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.
- Gene ID
- 18613 View all products for this Gene ID
- View information about CD31 on UniProt.org
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.