Purified anti-mouse CD28 Antibody

Pricing & Availability
Clone
37.51 (See other available formats)
Other Names
Tp44, T44
Isotype
Syrian Hamster IgG
Ave. Rating
2 reviews
Product Citations
publications
37.51
C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
  • 37.51
    C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
Cat # Size Price Quantity Avail. Save
102101 50 µg $45
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102102 500 µg $195
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Description

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD28 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102112). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102116) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
  2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
  3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
  4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
  5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
  6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
  8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
  9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
  10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  11. Barbi J, et al. 2007. Blood 110:2215.
  12. Milpied P, et al. 2011. Blood 118:2993. PubMed
  13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
  14. Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
  15. Li CR, et al. 2014. J Immunol. 192:1425. PubMed
  16. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. He N 2005. J Exp Med. 202:395. PubMed
  2. He N 2005. J Immunol. 174:6967. PubMed
  3. Perchonock C, et al. 2006. Mol Cell Biol . 26:6005. PubMed
  4. Perchonock C, et al. 2007. J Immunol. 179:1768. PubMed
  5. Rosenberger C, et al. 2008. Proc Natl Acad Sci U S A. 105:2544. PubMed
  6. Cunningham N, et al. 2011. Int Immunol. 1.439583333. PubMed
  7. Milpied P, et al. 2011. Blood. 118:2993. PubMed
  8. Crispin J, et al. 2012. J Immunol. 188:3567. PubMed
  9. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  10. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  11. McCully M, et al. 2015. J Immunol. 195: 96 - 104. PubMed
  12. Endo Y, et al. 2015. Cell Rep. 12: 1042-1055. PubMed
  13. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  14. Hollmann C, et al. 2016. J Immunol. 197: 3130 - 3141. PubMed
  15. Plaza-Sirvent C, et al. 2017. Cell Rep. 18(1):12-22. PubMed
  16. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  17. Kitamura T, et al. 2018. Front Immunol. 8:2004. PubMed
  18. Hao Y, et al. 2018. Front Immunol. 9:1127. PubMed
Publication Library
RRID
AB_312866 (BioLegend Cat. No. 102101)
AB_312867 (BioLegend Cat. No. 102102)

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
Function
Costimulates T and NK cells
Ligand Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
Thymocytes, T cells, NK cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.

Gene ID
12487 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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