Purified anti-MECP2 Antibody (Previously Covance catalog# MMS-5158)

Pricing & Availability
Clone
N227/21 (See other available formats)
Other Names
Methyl-CpG-binding protein 2, meCp-2 protein, RS, RTS, RTT, PPMX, MRX16, MRX79, MRXSL, AUTSX3, MRXS13
Previously
Covance Catalog# MMS-5158
Isotype
Mouse IgG1
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Product Citations
publications
1_N227slash21_PURE_MECP2_Antibody_IHC-P_1_112817
IHC staining of anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 1_N227slash21_PURE_MECP2_Antibody_IHC-P_1_112817
    IHC staining of anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2_N227slash21_PURE_MECP2_Antibody_IHC-P_2_112817
    IHC staining of anti-MECP2 antibody (clone N227/21) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N227slash21_PURE_MECP2_Antibody_ICC_1_112817
    ICC staining of anti-MECP2 antibody (clone N227/21) on HeLa cells. The cells were fixed with methanol, permeabilized with 0.1% Triton X-100 for 20 minutes, and blocked with 2% Normal Goat Serum and 0.02% BSA for 30 minutes. The cells were then incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 conjugated goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. The cells were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
  • N227slash21_PURE_MECP2_Antibody_ICC_2_112817
    ICC staining of anti-MECP2 antibody (clone N227/21) on mouse 3T3 cells. The cells were fixed with methanol, permeabilized with 0.1% Triton X-100 for 20 minutes, and blocked with 2% Normal Goat Serum and 0.02% BSA for 30 minutes. The cells were then incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 conjugated goat anti-mouse IgG (Cat. No. 405326) for one hour at room temperature. The cells were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
814001 100 µl $140.00
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Description

Methyl CpG binding protein (MECP2), also known as autism susceptibility to X-linked 2 (AUTSX3), is a nuclear protein crucial for normal neuronal function that binds to methylated DNA, forming complexes with histone deacteylases and turning off gene expression.  MECP2 expression is an X-linked trait. Loss of MECP2 expression causes Rett Syndrome (also known as RTS, RTT, or RS) a neurological disorder which causes decelerated growth of hands, feet, and the head.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 157-169 (FTVTG[dimethyl-R]GSPSRRE) of human MECP2.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. For immunocytochemistry, a concentration range of 1.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564780 (BioLegend Cat. No. 814001)

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Go To Top Version: 2    Revision Date: 06/23/2016

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