- 6C9 (See other available formats)
- Other Names
- LCA, T200
- Mouse IgG1
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- Product Citations
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CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.Product Details
- Antibody Type
- Host Species
- Marmoset mononuclear cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-F, WB - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen spleen sections1 and Western blotting1. The 6C9 antibody has been reported to not cross-react with humans, cynomolgus monkeys, or squirrel monkeys.
(PubMed link indicates BioLegend citation)
- Ito R, et al. 2008. Immunol. Lett. 121:116. (IHC, WB)
AB_2562119 (BioLegend Cat. No. 250202)
- Tyrosine phosphatases, type I transmembrane protein
Hematopoietic cells, except circulating erythrocytes or platelets
- TCR and BCR mediated activation
- Ligand Receptor
- Galectin-1, CD2, CD3, CD4
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
- Molecular Family
- CD Molecules
- Antigen References
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I and Thomas M. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 100385061 View all products for this Gene ID
- View information about CD45 on UniProt.org
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