Purified anti-MAG Antibody

Pricing & Availability
Clone
B11F7 (See other available formats)
Other Names
Siglec-4a, Myelin-associated Glycoprotein,
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
B11F7_Pure_MAG_Antibody_1_082718
Western blot of purified anti-MAG antibody (clone B11F7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of Hela cell lysate. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by the incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
  • B11F7_Pure_MAG_Antibody_1_082718
    Western blot of purified anti-MAG antibody (clone B11F7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of Hela cell lysate. The blot was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C, followed by the incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
  • B11F7_Pure_MAG_Antibody_2_082718
    Western blot of purified anti-MAG antibody (clone B11F7). Lane 1: Molecular weight marker; Lane 2: 20 µg of HEK293T cell lysate overexpressing human MAG; Lane 3: 20 µg of control HEK293T cell lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by the incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • B11F7_Pure_MAG_Antibody_3_082718
    IHC staining of purified anti-MAG antibody (clone B11F7) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B11F7_Pure_MAG_Antibody_4_082718
    IHC staining of purified anti-MAG antibody (clone B11F7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B11F7_Pure_MAG_Antibody_5_082718
    IHC staining of purified anti-MAG antibody (clone B11F7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
851701 25 µg $90
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851702 100 µg $235
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Description

Myelin-associated glycoprotein (MAG) is a cell membrane glycoprotein and a member of the siglec (sialic acid-binding immunoglobulin-like lectins) family. MAG has two isoforms in myelinated structures in the nervous system. MAG is expressed on oligodendrocytes and Schwann cells and is believed to be involved in myelination during nerve regeneration. MAG knockout mice develop quite severe pathological abnormalities associated with myelin instability.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified MAG protein isolated from human central nervous system.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Dobersen MJ, et al. 1985. Neurochem Research. 4: 499 (WB and IHC)
  2. Cao Z, et al. 2007. J Neurosci. 34: 9146 (WB)
RRID
AB_2749879 (BioLegend Cat. No. 851701)
AB_2749880 (BioLegend Cat. No. 851702)

Antigen Details

Structure
MAG is a 626 amino acid protein with a molecular mass of 70 kD.
Distribution

Tissue distribution: Central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures.
Cellular distribution: Plasma membrane

Function
MAG is involved in the process of axon myelination and maintenance of myelin sheath along axons. MAG also functions as a receptor that promotes differentiation, maintenance and survival of oligodendrocytes. Aberrant immunoreactivity to MAG has been suggested to play a role in the pathogenesis of multiple sclerosis.
Interaction
MAG binds to neuronal sialic acid-containing gangliosides and to glycoproteins RTN4R and RTN4RL2.
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Siglec Molecules
Antigen References
  1. Dobersen MJ, et al. 1985. Neurochem Research. 4: 499
  2. McKerracher L and Rosen KM. 2015. Front Mol Neurosci. 8: 51
  3. Vallat JM, et al. 2016. Expert Rev Neurother. 9: 1111
Gene ID
4099 View all products for this Gene ID
UniProt
View information about MAG on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/28/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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