Purified anti-LRP4 (NH2 terminus) Antibody (Previously Covance catalog# MMS-5154)

Pricing & Availability
Clone
N207/27 (See other available formats)
Other Names
Low-density lipoprotein receptor-related protein 4, LRP-4, LDLR dan
Previously
Covance Catalog# MMS-5154
Isotype
Mouse IgG2a
Ave. Rating
0 reviews
Product Citations
publications
N207slash27_PURE_LRP4_Anitbody_1_WB_032218
Western blot of purified anti-LRP4 (NH2 terminus) antibody (clone N207/27). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20ug of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N207slash27_PURE_LRP4_Anitbody_1_WB_032218
    Western blot of purified anti-LRP4 (NH2 terminus) antibody (clone N207/27). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20ug of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N207slash27_PURE_LRP4_Anitbody_2_IHC-P_032218
    IHC staining of purified anti-LRP4 (NH2 terminus) antibody (clone N207/27) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N207slash27_PURE_LRP4_Anitbody_3_IHC-P_032218
    IHC staining of purified anti-LRP4 (NH2 terminus) antibody (clone N207/27) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N207slash27_PURE_LRP4_Anitbody_4_IHC-P_032218
    IHC staining of purified anti-LRP4 (NH2 terminus) antibody (clone N207/27) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N207slash27_PURE_LRP4_Anitbody_5_IHC-P_032218
    IHC staining of purified anti-LRP4 (NH2 terminus) antibody (clone N207/27) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
832201 100 µl $140.00
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Description

Low-density lipoprotein receptor-related protein 4 (LRP4), also known as LRLR dan and multiple epidermal growth factor-like domains 7 (MEGF7), is a member of the LDLR family and has a large N terminus. LRP4 may help regulate Wnt signaling and is a receptor for agrin to activate muscle-specific kinase (MuSK), a critical tyrosine kinase in neuromuscular junction formation.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 26-350 (extracellular N-terminus) of mouse LRP4 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

RRID
AB_2564954 (BioLegend Cat. No. 832201)

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Go To Top Version: 2    Revision Date: 07/01/2016

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