Purified anti-Keratin 5 Polyclonal Chicken Antibody

Pricing & Availability
Clone
Poly9059 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type II cytoskeletal 5, CK-5, 58 kD cytokeratin, cytokeratin-5, type-II keratin Kb5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, keratin 5 (epidermolysis bullosa simplex), krt5
Isotype
Chicken IgY
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Product Citations
publications
Poly9059_PURE_Keratin5_antibody_102517
IHC staining of anti-Keratin 5 antibody (Poly90590) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate (Cat. No. 928602), the tissue was incubated with the primary antibody for 60 minutes at room temperature at 1 µg/ml. After incubation with biotinylated anti-chicken antibody, the tissue was labeled with BioLegend’s HRP labeling reagent followed by addition of AEC chromogen (Cat. No. 925804). The tissue was then counterstained with hematoxylin.
  • Poly9059_PURE_Keratin5_antibody_102517
    IHC staining of anti-Keratin 5 antibody (Poly90590) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate (Cat. No. 928602), the tissue was incubated with the primary antibody for 60 minutes at room temperature at 1 µg/ml. After incubation with biotinylated anti-chicken antibody, the tissue was labeled with BioLegend’s HRP labeling reagent followed by addition of AEC chromogen (Cat. No. 925804). The tissue was then counterstained with hematoxylin.
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905903 25 µg $143
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905904 100 µg $357
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Description

Keratin 5 belongs to the type II cytokeratin family of proteins. It dimerizes with Keratin 14 to form intermediate filaments (IFs) that make up the cytoskeleton of the basal epithelial cells. Keratin 5 is involved in several diseases including breast and lung cancers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 5 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 – 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Yuan T, et al. 2020. Front Pharmacol. 11:120. PubMed
  2. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
RRID
AB_2721742 (BioLegend Cat. No. 905903)
AB_2721743 (BioLegend Cat. No. 905904)

Antigen Details

Structure
Keratin 5 is a 590 amino acid protein with a molecular mass of 62 kD.
Distribution

Tissue distribution: esophagus, hair follicle, saliva, and skin.
Cellular distribution: cytoskeleton, extracellular, nucleus, and cytosol.

Function
Keratin 5 IFs contribute to the cell architecture and provide the cells with elasticity to withstand mechanical and non-mechanical stresses.
Interaction
Keratin 14
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References
  1. Yuspa SH, et al 1989 J Cell Biol 109:1207.
  2. Roop, DR, et al 1984 J Biol Chem 259:8037.
Gene ID
3852 View all products for this Gene ID
UniProt
View information about Keratin 5 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 09/25/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
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  • Keratin 5 Polyclonal Chicken Antibody, Purified

  • Purified anti-Keratin 5 Polyclonal Chicken

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