Purified anti-Keratin 14 Antibody

Pricing & Availability
Clone
Poly9060 (See other available formats)
Other Names
Keratin, type I cytoskeletal 14, CK-14, cytokeratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner), K14, CK14
Isotype
Chicken Polyclonal IgY
Ave. Rating
2 reviews
Product Citations
publications
1_Poly9060_PURE_Keratin14_Antibody_1_IHCP_062216
IHC staining of anti-Keratin 14 antibody (Poly9060) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. After incubation with biotinylated anti-chicken antibody, the tissue was labeled with BioLegend’s HRP labeling reagent followed by addition of red chromogen. The tissue was then counterstained with hematoxylin.
  • 1_Poly9060_PURE_Keratin14_Antibody_1_IHCP_062216
    IHC staining of anti-Keratin 14 antibody (Poly9060) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. After incubation with biotinylated anti-chicken antibody, the tissue was labeled with BioLegend’s HRP labeling reagent followed by addition of red chromogen. The tissue was then counterstained with hematoxylin.
  • 2_Poly9060_PURE_Keratin14_Antibody_1_WB_112817
    Western blot analysis of cell lysates from HaCaT (positive control), THP-1 (low expression negative control) and UMR106 (Rat) cells using Keratin 14 Chicken primary antibody (clone Poly19060, 1:500 dilution) and HRP Donkey anti-chicken secondary antibody (1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control (1:8000 dilution).
  • 3_Poly9060_PURE_Keratin14_Antibody_2_WB_062216
    Western Blot of anti-Keratin 14 antibody (Poly9060). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 1 µg/ml for 24 hours at 2-8°C followed by detection with a fluorescent labeled goat anti-chicken secondary antibody.
  • Poly9060_PURE_Keratin-14_Antibody_ICC_122117
    HaCaT cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Chicken IgY isotype control (Negative, Cat. No. 402101) or (B-D) Keratin 14 primary antibody (Clone Poly9060) overnight at 4°C followed by Alexa Fluor® 488 (Green) Goat anti-chicken IgY incubation for one hour at room temperature. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/30. Concentrations for (A-B) is 5 µg/ml (1:100 dilution), (C) is 2 µg/ml (1:250 dilution) and (D) is 1 µg/ml (1:500 dilution).
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Cat # Size Price Quantity Avail. Save
906004 100 µg $245
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Description

Keratin 14 is a member of the keratin family, which is the most diverse group of intermediate filaments. Keratin 14 is a type I keratin, and is  usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, Cross-Reactivity: Canine, Primate
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 14 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemisty, a concentration range of 0.1 - 1.0 μg/ml is suggested. For immunocytochemistry, a concentration range of 1.0 - 2.0 μg/ml (1:500-1:250) is recommended. For Western blotting, the suggested usage is 1.0 µg/ml (1:500). It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Yuspa SH, et al. 1989. J. Cell Biol. 109:1207.
  2. Roop RD, et al. 1984. J. Biol. Chem. 259:8037.
Product Citations
  1. Ding X, et al. 2016. Nat Commun. 7:13226. PubMed
  2. Tripurani SK, et al. 2018. Mol Biol Cell. 29:2784. PubMed
  3. O'Rourke BP, et al. 2019. Adv Wound Care (New Rochelle). 8:91. PubMed
Publication Library
RRID
AB_2616962 (BioLegend Cat. No. 906004)

Antigen Details

Structure
Keratin 14 is a 52 kD protein.
Distribution

Cytoplasm, extracellular, and nucleus.

Function
Nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles, enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3861 View all products for this Gene ID
UniProt
View information about Keratin 14 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 11/28/2017

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