Purified anti-KCC2 Antibody (Previously Covance catalog# MMS-5241)

Pricing & Availability
Clone
N1/12 (See other available formats)
Other Names
SLC12A5, Solute carrier family 12 member 5, rKCC2, K-Cl cotransporter 2, neuronal K-Cl cotransporter, solute carrier family 12, member 5, neuronal-specific K-Cl cotransporter, furosemide-sensitive K-Cl cotransporter, electroneutral potassium-chloride cotransporter 2
Previously
Covance Catalog# MMS-5241
Isotype
Mouse IgG2a
Ave. Rating
0 reviews
N1slash12_Purified_KCC2_Antibody_1_021918
Western blot of purified anti-KCC2 antibody (clone LN1/12). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N1slash12_Purified_KCC2_Antibody_1_021918
    Western blot of purified anti-KCC2 antibody (clone LN1/12). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N1slash12_Purified_KCC2_Antibody_2_021918
    IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N1slash12_Purified_KCC2_Antibody_3_021918
    IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N1slash12_Purified_KCC2_Antibody_4_021918
    IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
821701 100 µl $140.00
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Description

Potassium-chloride cotransporter member 5 (also known as KCC2 and SCL12A5) is responsible for establishing the chloride ion gradient in neurons through the maintenance of low intracellular chloride concentrations.  Mediates synaptic inhibition, protects against excitotoxicity and modulates plasticity.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).

Application References

(PubMed link indicates BioLegend citation)
  1. Hartmann A, et al. 2017. PLoS One. 12(6):e0179968. PubMed
Publication Library
RRID
AB_2564853 (BioLegend Cat. No. 821701)

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Go To Top Version: 3    Revision Date: 06/23/2016

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