- Clone
- N1/12 (See other available formats)
- Other Names
- SLC12A5, Solute carrier family 12 member 5, rKCC2, K-Cl cotransporter 2, neuronal K-Cl cotransporter, solute carrier family 12, member 5, neuronal-specific K-Cl cotransporter, furosemide-sensitive K-Cl cotransporter, electroneutral potassium-chloride cotransporter 2
- Previously
-
Covance Catalog# MMS-5241
- Isotype
- Mouse IgG2a
- Ave. Rating
- 0 reviews
-
Western blot of purified anti-KCC2 antibody (clone LN1/12). Lane 1: Molecular weight marker; Lane 2: 30 µg of mouse brain membrane lysate; Lane 3: 30 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-KCC2 antibody (clone LN1/12) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 821701 | 100 µl | $140.00 | ||||
Potassium-chloride cotransporter member 5 (also known as KCC2 and SCL12A5) is responsible for establishing the chloride ion gradient in neurons through the maintenance of low intracellular chloride concentrations. Mediates synaptic inhibition, protects against excitotoxicity and modulates plasticity.
Product DetailsProduct Details
- Reactivity
- Mouse, Rat
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a fusion protein corresponding to amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2b.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
WB - Quality tested
IHC-P - Validated - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and formalin-fixed paraffin-embedded immunohistochemical staining (IHC-P).
-
Application References
(PubMed link indicates BioLegend citation) -
- Hartmann A, et al. 2017. PLoS One. 12(6):e0179968. PubMed
- RRID
-
AB_2564853 (BioLegend Cat. No. 821701)
Antigen Details
- Structure
- Expected MW: 140 kD
- Gene ID
- 171373 View all products for this Gene ID
- UniProt
- View information about KCC2 on UniProt.org
Related FAQs
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.