- 5A3A39 (See other available formats)
- Regulatory Status
- Other Names
- Interferon regulatory factor 9 (IRF-9), Interferon-stimulated gene factor 3 gamma (ISGF-3 gamma), ISGF3 p48 subunit
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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IRF9 is a member of the IRF family of transcription factors and plays an important role in IFN signaling pathways and anti-viral immune responses. Type I and type III interferon stimulation induces phosphorylation and heterodimerization of STAT1 and STAT2. The activated STAT1:STAT2 heterodimer recruits IRF9 and forms a heterotrimeric complex termed IFN-stimulated gene factor 3 (ISGF3). The ISGF3 complex, in turn, translocates to the nucleus and transactivates IFN-inducible genes. The IRF9 protein in the ISGF3 complex is responsible for the recognition of IFN-stimulated response elements (ISRE). Mice lacking IRF9 fail to induce the expression of IFN regulated genes in response to type I interferon stimulation, indicating that IRF9 is crucial in mediating interferon signaling.Product Details
- Antibody Type
- Host Species
- Partial human IRF9 recombinant protein (116-387 a.a.) expressed in E. coli.
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Affinity purified.
- 0.5 mg/mL
- Storage & Handling
- Upon receipt, store undiluted 2°C and 8°C.
WB - Quality tested
ICC, ChIP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.4 - 2.5 µg per mL. For ChIP applications, the suggested dilution is 1:50 to 1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone does not react with mouse (in-house tested). A non-specific band was observed (around 37 KD) when this antibody was tested on Raw264.7 cells
- Product Citations
AB_2563575 (BioLegend Cat. No. 660702)
- 393 amino acids, predicted molecular weight of 44 kD; contains a IRF tryptophan pentad repeat responsible for DNA binding.
Cytoplasm, phosphorylated form translocated to the nucleus.
- IRF9 is a transcription factor that mediates interferon signaling and activates the expression of interferon inducible genes.
- Forms the interferon-stimulated gene factor 3 complex (ISGF3) with Signal Transducer and Activator of Transcription 1 (STAT1) and STAT2 in response to type I IFN stimulation.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Fink K and Grandvaux N, 2013. JAKSTAT. 2:e27521.
2. Tsuno T, et al. 2009. J. Immunother. 32:803.
3. Thibault DL, et al. 2008. J. Clin. Invest. 118:1417.
4. Kraus TA, et al. 2003. J. Biol. Chem. 278:13033.
5. Xiao W, et al. 2001. J. Biol. Chem. 276:23275.
6. Veals SA, et al. 1992. Mol. Cell. Biol. 12:3315.
- Gene ID
- 10379 View all products for this Gene ID
- View information about IRF9 on UniProt.org
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