- W16070A (See other available formats)
- Other Names
- NF-kappa-B essential modulator, FIP-3, IKKAP1, IKK-gamma, IKKG, IKBKG
- Rat IgG2a, κ
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- Product Citations
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NF-kB represents a family of transcription factors that are normally inactive in the cytoplasm by binding inhibitory molecules of the IkB proteins. In response to various stimuli such as: inflammatory cytokines, bacterial or viral products, or various types of stress, the IkB proteins become phosphorylated by Iκ B kinase (IKK) complex. This leads to the polyubiquitination and destruction by the proteasome. Consequently, free NF-kB enters the nucleus and activates transcription of a variety of genes involved in immune and inflammatory response, cell adhesion, growth control, and inhibition of apoptosis. The IKK complex consists of the regulatory subunit IKKγ/NEMO (NF-κ B Essential Modifier) and two catalytic subunits (IKKα and IKKβ). IKKγ is an adaptor protein involved in activation of the IKK kinases and has been shown to be essential for the canonical and stress-induced NF-κ B pathway. Recently, Medunjanin S, et al reported that GSK-3β is critically important for ordered NF-κB signaling through modulation of IKKγ phosphorylation.
IKKγ deficiency in mice resulted in a lack of detectable NF-κ B DNA-binding activity and a lethal embryonic phenotype because of severe liver damage due to massive apoptosis. Two distinct X-linked human diseases, incontinentia pigmenti (IP) and anhidrotic ectodermal dysplasia associated with immunodeficiency (EDA-ID), have been linked to IKKγ dysfunction; indicating the critical roles of IKKγ in human development, skin homeostasis, and innate/acquired immunity.Product Details
- Antibody Type
- Host Species
- Partial human IKKγ (240-419 a.a.) expressed in E. coli.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg per ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone W16070A does not cross-react with mouse (in-house tested).
AB_2650723 (BioLegend Cat. No. 694101)
AB_2650724 (BioLegend Cat. No. 694102)
- 419 amino acids, and the predicted molecular weight is 48 kD.
- Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B, thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor.
- Component of the IKK core complex consisting of CHUK, IKBKB and IKBKG. Interacts with COPS3, CYLD, NALP2, TRPC4AP and LRDD. Interacts with ATM, TRAF6, IKBKE, TANK, ZFAND5, RIPK2, TNIP1 and TNFAIP3, polyubiquitin, USP10, ZC3H12A.
- Biology Area
- Cell Biology, Signal Transduction
- Antigen References
1. Israël A. 2010. Cold Spring Harb. Perspect. Biol. 2:a000158
2. Rothwarf DM, et al. 1998. Nature. 395:297.
3. Medunjanin S, et al. 2016. Sci. Rep. 6:38553.
4. Rudolph D, et al. 2000. Genes. Dev. 14:854
5. Smahi A, et al. 2000. Nature. 405: 466
6. Fusco F, et al. 2008. Hum. Mutat. 29: 595
- Gene ID
- 8517 View all products for this Gene ID
- View information about IKKgamma on UniProt.org
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