Purified anti-human M-CSF Antibody

Pricing & Availability
Clone
L168B11 (See other available formats)
Other Names
Colony Stimulating Factor 1 (Macrophage), Macrophage Colony-Stimulating Factor, Lanimostim, CSF1, CSF-1, MCSF
Isotype
Mouse IgG3, κ
Ave. Rating
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Product Citations
publications
L168B11_MCSF_Purified_Human_WB_081814.jpg
Immunoprecipitation/Western blot analysis using the M-CSF antibody (L168B11). Supernatant of M-CSF overexpressing cells was tested at a 1:40 dilution. Lane 1 was immunoprecipitated with mouse IgG control antibody and lane 2 was immunoprecipitated with M-CSF antibody (L168B11). Western blot analysis was performed using goat anti-human M-CSF antibody.
  • L168B11_MCSF_Purified_Human_WB_081814.jpg
    Immunoprecipitation/Western blot analysis using the M-CSF antibody (L168B11). Supernatant of M-CSF overexpressing cells was tested at a 1:40 dilution. Lane 1 was immunoprecipitated with mouse IgG control antibody and lane 2 was immunoprecipitated with M-CSF antibody (L168B11). Western blot analysis was performed using goat anti-human M-CSF antibody.
Cat # Size Price Quantity Avail. Save
661602 500 µg $325
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Description

M-CSF is a cytokine that controls the development, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer. M-CSF is released by fibroblasts, breast cancer cell lines, alveolar macrophages, stromal bone marrow cells, endothelial cells, and mesenchymal cells. It binds CD14+ monocytes and promotes the survival/proliferation of human peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions, including phagocytic activity, microbial killing, cytotoxicity against tumor cells as well as synthesis of inflammatory cytokines such as IL-1β, TNF-α, and IFN-γ in monocytes. M-CSF induces RANKL production in mature human osteoclasts; consequently, M-CSF is a potent stimulator of mature osteoclast resorbing activity. Also, M-CSF induces monocyte-derived VEGF in human tumors. High levels of M-CSF, mononuclear phagocytes, and VEGF are associated with poor prognosis in patients with cancer.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human M-CSF
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IP - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunoprecipitation. For immunoprecipitation, the suggested use of this reagent is 2-10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564049 (BioLegend Cat. No. 661602)

Antigen Details

Structure
Three isoforms with 256-554 amino acids, predicted molecular weight of approximately 30-70 kD.
Distribution

Cell membrane as a single-pass membrane protein. Processed macrophage colony-stimulating factor 1 is secreted into the extracellular space.

Function
Controls the production, differentiation, and function of macrophages.
Interaction
Disulfide-linked homodimer or heterodimer that interacts with CSF1R.
Ligand/Receptor
M-CSFR or CSF1R (CD115).
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Kawasaki ES, et al. 1985. Science 230:291.
2. Wei S, et al. 2010. J. Leukoc. Biol. 88:495.
3. Hodge JM, et al. 2011. PloS One 6:e21462.
4. Morandi A, et al. 2011. PloS One 6:e27450.
5. Erblich B, et al. 2011. PloS One 6:e26317.
6. MacDonald KP, et al. 2010. Blood 116:3955.

Gene ID
1435 View all products for this Gene ID
UniProt
View information about M-CSF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/28/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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