Purified anti-human IL-31 Antibody

Pricing & Availability
Clone
Poly5300 (See other available formats)
Other Names
Interleukin-31,
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Poly5300_PURE_IL-31_Antibody_032717
This standard curve was generated at BioLegend for demonstration purposes only. Overnight incubation for standard and samples at 4°C is recommended. For detection antibody, two hour incubation with shaking is recommended.
  • Poly5300_PURE_IL-31_Antibody_032717
    This standard curve was generated at BioLegend for demonstration purposes only. Overnight incubation for standard and samples at 4°C is recommended. For detection antibody, two hour incubation with shaking is recommended.
Cat # Size Price Quantity Avail. Save
530002 100 µg $225
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Description

IL-31, produced mainly by activated CD4+ T cells, is a newly discovered helical cytokine belonging to the gp130/IL6 cytokine family. IL-31 is closely related in structure to IL-6, IL-11, and IL-27. Binding of IL-31 to its receptor activates JAK/STAT, PI3K/AKT, and MAPK pathways. IL-31 acts on a broad range of immune and non-immune cells and thus possesses potential pleiotropic physiological functions, such as regulating hematopoiesis and immune responses. Human IL-31 is also involved in inflammatory bowel disease, airway hypersensitivity, and dermatitis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human IL-31
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-31 recombinant protein ranging from 2000 to 31.3 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

The purified Poly5300 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5300 antibody as the detecting antibody. 

RRID
AB_2650707 (BioLegend Cat. No. 530002)

Antigen Details

Structure
161 aa with a putative 23 aa signal peptide that is cleaved to generate a 138 aa mature protein.
Distribution

Secreted by activated CD4<sup>+</sup> T cells.

Function
IL-31 belongs to the IL-6 family of cytokines and plays a role in skin inflammation.
Ligand Receptor
Binds to IL-31 receptor complex that consists of two receptor subunits, IL31RA and OSMR.
Cell Type
T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. McCandless EE, et al. 2014. Vet. Immunol. Immunopathol. 157:42.
2. Arai I, et al. 2013. Exp. Dermatol. 22:669.
3. Dai X, et al. 2013. PLoS One. 8:e67666.
4. Stott B, et al. 2013. J. Allergy Clin. Immunol. 132:446.
5. Hartmann K, et al. 2013. J. Allergy Clin. Immunol. 132:232.
6. Baron JM and Lüscher B. 2012. Acta. Derm. Venereol. 92:5.

Gene ID
386653 View all products for this Gene ID
UniProt
View information about IL-31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03/28/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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