Purified anti-human IL-22 Antibody

Pricing & Availability
Clone
Poly5161 (See other available formats)
Other Names
TIFa, IL-D110, IL-TIF, TIFIL-23, zcyto18
Isotype
Goat Polyclonal Ig
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516101 100 µg $225
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Description

IL-22 is a cytokine structurally related to IL-10. Originally identified as a murine gene induced by IL-9 in T and mast cells, IL-22 was initially designated ILTIF (IL-10-related T cell-derived inducible factor).   IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL-20, IL-24, IL-26, and the IFN-λs, IL-28A, IL-28B and IL-29.  Human IL-22 shares 79% amino acid identity with murine IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains. Activity is further regulated through interactions with the soluble binding protein, IL-22BP, which shares sequence similarity with an extracellular region of IL-22R1 (sIL-22R1).  Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by Th17 cells and newly identified Th22 cells. It plays a critical role in mucosal immunity in addition to the deregulated inflammation observed in autoimmune diseases.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Human IL-22, amino acids Ala34-Ile179 (Accession# NM_020525) was expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by antigen affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-22 recombinant protein ranging from 8000-125 pg/ml are recommended.

Application Notes

ELISA Capture: The purified Poly5161 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with biotinylated Poly5161 antibody (Cat. No. 516103) as the detecting antibody and recombinant human IL-22 (Cat. No. 571309) as the standard.

RRID
AB_2561330 (BioLegend Cat. No. 516101)

Antigen Details

Structure
Cytokine
Distribution
Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells, Th17
Function
Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; Promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Interaction
Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Ligand Receptor
IL-22R1 and IL-10R2 receptor chains
Cross
In sandwich ELISA, less than 10% cross-reactivity with recombinant mouse IL-22.
Cell Type
T cells, Macrophages, Th17, Mast cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagalakshmi ML, et al. 2004. Intern. Immunopharmacol. 4:679.
2. Kebir H, et al. 2007. Nat. Med. 13:1173.
3. Gu Y, et al. 2008. Eur. J. Immunol. 38:1807.
4. Pene J, et al. 2008. J. Immunol. 180:7423.
5. Dumotier L, et al. 2000. J. Immune. 164:1814.
6. Xie MH, et al. 2000. J. Biol. Chem. 765:31335.
7. Kotenko SV, et al. 2001. J. Immunol. 166:7096.
8. Chang C, et al. 2006. Cell Res. 16:902.
9. Trifari S,et al. 2009. Nat. Immunol. 10:864.

Gene ID
50616 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

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