Purified anti-human IL-15 Antibody

Pricing & Availability
Clone
BH1509 (See other available formats)
Other Names
Interleukin-15 (IL-15), MGC9721
Isotype
Mouse IgG1, κ
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Cat # Size Price Quantity Avail. Save
515001 50 µg $90
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515002 500 µg $240
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Description

Interleukin-15 (IL-15) was discovered in supernatant from simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2 (1). IL-15 is a regulatory cytokine that is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts, and monocytes (2). It plays an important role in immune response and shares many functions with IL-2, for example, it stimulates the proliferation of activated T cells (1, 2), NK cells (3) and B cells, and it induces immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand (4). In addition, IL-15 promotes the development of dendritic cells (5), activates human neutrophils (6, 7) and induces the production of proinflammatory cytokines from macrophages (8). IL-15 acts as a bridge between innate and adaptive immunity because of its diverse roles in the immune system. IL-15 binds to heterotrimeric receptors composed of IL-15Rα, IL-15Rβ, and IL-15Rγc. IL-15 shares with IL-2 the receptor chains β and γc. IL-15 is normally not secreted in soluble form but is held on the cell surface bound to a unique receptor, IL-15Rα, especially on dendritic cells. Cell-bound IL-15 then is presented in trans to T cells and NK cells and is recognized by the γc receptor on these cells; such recognition maintains cell survival and intermittent proliferation (9).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli - expressed, recombinant human IL-15
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-15 recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified BH1509 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with biotinylated BH1543 antibody (Cat. No. 515104) as the detecting antibody and recombinant human IL-15 (Cat. No. 570309) as the standard.

RRID
AB_2561318 (BioLegend Cat. No. 515001)
AB_2561319 (BioLegend Cat. No. 515002)

Antigen Details

Structure
13 kD single non-glycosylated polypeptide
Distribution
T cells, B cells, dendritic cells, monocytes, and macrophages
Function
IL-15 stimulates the proliferation of activated T cells, NK cells, and B cells, inducing immunoglobulin synthesis by B cells stimulated by anti-IgM or CD40 ligand. In addition, IL-15 promotes the development of dendritic cells, activates human neutrophils, and induces the production of proinflammatory cytokines from macrophages.
Interaction
T lymphocytes, NK cells
Ligand/Receptor
IL-15Rα, IL-15Rβ and IL-15γc
Cell Type
T cells, B cells, Dendritic cells, Monocytes, Macrophages
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Grabstein K, et al. 1994. Science 264:965.
2. Ma A, et al. 2006. Annu. Rev. Immunol. 24:657.
3. Meresse B, et al. 2004. Immunity 21:357.
4. Armitage RJ, et al. 1995. J. Immunol. 154:483.
5. Pulendran B, et al. 2004. Eur. J. Immunol. 34:66.
6. Bouchard A, et al. 2004. J. Leuko. Biol. 75:893.
7. Ratthe C, et al. 2004. J. Leuko. Biol. 76:162.
8. Tang F, et al. 2008. Cell Immunol. 5:189.
9. Rubinstein MP, et al. 2006. P. Natl. Acad. Sci. USA 103:9166.

Gene ID
3600 View all products for this Gene ID
UniProt
View information about IL-15 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/06/2013

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