Purified anti-human IFN-γ Antibody

Pricing & Availability
Clone
MD-1 (See other available formats)
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
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publications
Cat # Size Price Quantity Avail. Save
507501 50 µg $90
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507502 500 µg $240
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The 4S.B3 antibody reacts with the human IFN-γ. The MD-1 antibody reacts with human and monkey interferon-gamma (IFN-γ). The MD-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity*: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
ICFC, IHC-F, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, the antibody should be titrated between 0.25 - 2 µg/ml to determine optimal condition. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1,2,5 and ELISPOT3,4 Capture: The Purified MD-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4S.B3 (Cat. No. 502504/502514) antibody as the detecting antibody and recombinant human IFN-γ (Cat. No. 570209) as the standard. The LEAF™ Purified antibody is suggested for ELISPOT capture. For ELISPOT capture applications, a concentration range of 0.5-2 µg/ml is recommended.
Neutralization1: The LEAF™ Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IFN-γ bioactivity (Cat. No. 507513).
Additional reported applications (for the relevant formats) include: Western blotting1,2, intracellular staining for flow cytometry6, and immunohistochemical staining7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Van der Meide P, et al. 1985. J. Immuno. Meth. 79:293.
  2. Yazdanbakhsh M, et al. 1993. Eur. J. Immuno. 23:3312.
  3. Besouw N, et al. 1994. J. Med. Primatol. 23:42.
  4. Van der Meide P, et al. 1995. J. Med. Primatol. 24:271.
  5. Hilkens C, et al. 1996. J. Immunol. 156:1722.
  6. Hamann D, et al. 1996. J. Immunol. 156:1387.
  7. Wassenaar A, et al. 1995. Infect. Immunity. 63:2147.
RRID
AB_2122340 (BioLegend Cat. No. 507501)
AB_2122339 (BioLegend Cat. No. 507502)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 05/23/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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