Purified anti-human HLA-DR Antibody

Pricing & Availability
Clone
Tü36 (See other available formats)
Other Names
MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
Tu36_Purified_HLA-DR_Antibody_FC_012314.jpg
Human peripheral blood lymphocytes were stained with purified HLA-DR (clone Tü36) (filled histogram) or mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • Tu36_Purified_HLA-DR_Antibody_FC_012314.jpg
    Human peripheral blood lymphocytes were stained with purified HLA-DR (clone Tü36) (filled histogram) or mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
Cat # Size Price Quantity Avail. Save
361602 100 µg $105
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Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of an α (heavy) chain and a β (light) chain. They are expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Variations in the HLA gene expression are crucial to graft survival.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Baboon, Cynomolgus, Cat (Feline), Dog (Canine), Cattle (Bovine, Cow)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (of relevant formats) includes Western blotting4, immunoprecipitation4, and in vitro blocking5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Pawelec G, et al. 1985. Hum. Immunol. 12:165. (FC)
  2. Shaw S, et al. 1985. Hum. Immunol. 12:191. (FC)
  3. Ziegler A, et al. 1986. Immunobiology. 171:77. (FC)
  4. Cebulla CM, et al. 2002. J. Immunol. 169:167. (WB, FC, IP)
  5. Khaw LT, et al. 2013. PLOS One. 8:e69521. (Block)
RRID
AB_2563138 (BioLegend Cat. No. 361602)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Thorsby E. 1974. Transplant. Rev. 18:51.
2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207.
3. Servenius B, et al. 1984. EMBO J. 3:3209.
4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105.
5. Strassmann G, et al. 1985. Hum. Immunol. 13:125.
6. Trowsdale J, et al. 1985. Immunol. Rev. 85:5.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID 3113 View all products for this Gene ID 3115 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 11/07/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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