- Tü36 (See other available formats)
- Other Names
- MHC class II, Major Histocompatibility complex II, human leukocyte antigen, HLA
- Mouse IgG2b, κ
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- Product Citations
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HLA-DR is a heterodimeric cell surface glycoprotein comprised of an α (heavy) chain and a β (light) chain. They are expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. Variations in the HLA gene expression are crucial to graft survival.Product Details
- Human, Cross-Reactivity: Baboon, Cynomolgus, Cat (Feline), Dog (Canine), Cattle (Bovine, Cow)
- Antibody Type
- Host Species
- Human PBL
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IP, WB - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (of relevant formats) includes Western blotting4, immunoprecipitation4, and in vitro blocking5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (contact our custom solutions team).
(PubMed link indicates BioLegend citation)
- Pawelec G, et al. 1985. Hum. Immunol. 12:165. (FC)
- Shaw S, et al. 1985. Hum. Immunol. 12:191. (FC)
- Ziegler A, et al. 1986. Immunobiology. 171:77. (FC)
- Cebulla CM, et al. 2002. J. Immunol. 169:167. (WB, FC, IP)
- Khaw LT, et al. 2013. PLOS One. 8:e69521. (Block)
AB_2563138 (BioLegend Cat. No. 361602)
- Ig superfamily, MHC class II, heterodimeric transmembrane protein
B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs
- Antigen presentation
- Ligand Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Thorsby E. 1974. Transplant. Rev. 18:51.
2. Qvigstad E, et al. 1984. Hum. Immunol. 11:207.
3. Servenius B, et al. 1984. EMBO J. 3:3209.
4. Ottenhoff TH, et al. 1985. Hum. Immunol. 13:105.
5. Strassmann G, et al. 1985. Hum. Immunol. 13:125.
6. Trowsdale J, et al. 1985. Immunol. Rev. 85:5.
- Gene ID
- 3122 View all products for this Gene ID 3123 View all products for this Gene ID 3113 View all products for this Gene ID 3115 View all products for this Gene ID
- View information about HLA-DR on UniProt.org
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