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Purified anti-human CD68 Antibody

Pricing & Availability
Clone
BL13756 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Mouse IgG1, κ
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Product Citations
publications
BL13756_PURE_CD68_Antibody_083120.png
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes and permeabilized with 0.5% Triton X-100. Then, the tissue was stained with 10 µg/mL of purified anti-human CD68 (clone BL13756) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse secondary antibody (Poly4053) Alexa Fluor™ 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
  • BL13756_PURE_CD68_Antibody_083120.png
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate buffer 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, blocked with 5% FBS and 0.2% gelatin for 30 minutes and permeabilized with 0.5% Triton X-100. Then, the tissue was stained with 10 µg/mL of purified anti-human CD68 (clone BL13756) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with goat anti-mouse secondary antibody (Poly4053) Alexa Fluor™ 594 (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was scanned with a 10X objective and stitched with software.
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375602 100 µg $112
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Description

CD68 is a 110 kD glycoprotein, also known as macrosialin, belonging to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to residues from the internal region of the human CD68 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2876705 (BioLegend Cat. No. 375602)

Antigen Details

Structure
Sialomucin family, 110 kD
Distribution

Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells

Molecular Family
CD Molecules
Antigen References

1. Holness CL and Simmons DL. 1993. Blood. 81:1607.
2. Gottfried E, et al. 2008. Scand. J. Immunol. 67:453.
3. Hameed A, et al. 1994. Hum. Pathol. 25:872.

Gene ID
968 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/31/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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