Purified anti-human CD66b Antibody

Pricing & Availability
6/40c (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
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Product Citations
Human peripheral blood granulocytes was stained with CD66b (clone 6/40c) purified (filled histogram) or mouse IgG1, κ isotype control (clone MOPC-21) purified (open histogram) followed by Poly4053 PE.
  • 6slash40c_pure_CD66b_Antibody_012918
    Human peripheral blood granulocytes was stained with CD66b (clone 6/40c) purified (filled histogram) or mouse IgG1, κ isotype control (clone MOPC-21) purified (open histogram) followed by Poly4053 PE.
  • 6slash40c_PURE_CD66b_Antibody_IHC-P_032018
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R., 1x pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD66b (clone 6/40c) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 Goat anti-mouse IgG (clone poly4053) antibody (red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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392902 100 µg $141
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CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant soluble human CEACAM8-Fc produced in HEK293 cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Singer, et al. 2014. PLoS One. 9(4):e94106. PMID: 24743304
  2. Klaile, et al. 2013. Respir Res. 14:85. PMID 23941132
Product Citations
  1. He M, et al. 2021. J Gynecol Oncol. e32:32. PubMed
  2. Riquelme E et al. 2019. Cell. 178(4):795-806 . PubMed
  3. Zheng Y, et al. 2022. iScience. 25:103785. PubMed
AB_2728422 (BioLegend Cat. No. 392902)

Antigen Details

Ig superfamily, CEA antigen group, GPI-linked glycoprotein


Cell adhesion, neutrophil activation
Cell Type
Biology Area
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Kuijpers T, et al. 1993. J Immunol. 151:4934.
  2. Kuroki M, et al. 1992. J Leuk Biol. 52:551.
Gene ID
1088 View all products for this Gene ID
View information about CD66b on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01/30/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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