Purified anti-human CD303 (BDCA-2) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
201A (See other available formats)
Other Names
CLEC4C, CLECSF11, CLECSF7, DLEC, HECL
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
201A_Purified_CD303_Cytof_060414.jpg
Human PBMCs stained with 151Eu-anti-CD123 (6H6) and 153Eu-anti-CD303 (201A). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.
  • 201A_Purified_CD303_Cytof_060414.jpg
    Human PBMCs stained with 151Eu-anti-CD123 (6H6) and 153Eu-anti-CD303 (201A). Lymphocytes are displayed in the analysis. Data provided by DVS Sciences.
Cat # Size Price Quantity Avail. Save
354215 100 µg $220
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Description

CD303, also known as BDCA-2 and CLEC4C, is a 38 kD type II transmembrane glycoprotein. It is a member of the C-type lectin superfamily. CD303 is expressed by plasmacytoid dendritic cells (pDCs) and is involved in cell adhesion, signaling, and antigen capture and processing. Crosslinking of CD303 inhibits the production of IFN-α/β and TLR-9 induced pDCs maturation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Validated

Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated so that the buffer exchange step is not required (steps 7, 8, 9, and 10 in the Maxpar® antibody labeling protocol). Just add 100 µl of this antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter, as described in step 11, and continue with the protocol.
Additional Product Notes
Maxpar® is a registered trademark of Fluidigm Inc.
Application References

(PubMed link indicates BioLegend citation)
  1. Freeman A, et al. 2014. PLoS One. 9:110928. PubMed
  2. Johnson P, et al. 2015. Clin Cnacer Res. 21:1321. PubMed
RRID
AB_2563739 (BioLegend Cat. No. 354215)

Antigen Details

Structure
Type II transmembrane protein, 38 kD, member of the C-type lectin superfamily
Distribution

Plasmacytoid dendritic cells

Function
Antigen capture, inhibition of IFN α/β production
Cell Type
Dendritic cells
Biology Area
Immunology, Innate Immunity, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Jähn PS, et al. 2010. Cell Immunol. 265:15.
2. Graham LM and Brown GD. 2009. Cytokine 48:148.
3. Röck J, et al. 2007. Eur. J. Immunol. 37:3564.
4. Dzionek A, et al. 2001. J. Exp. Med. 194:1823.

Gene ID
170482 View all products for this Gene ID
UniProt
View information about CD303 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 0    Revision Date: 07/30/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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