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Purified anti-human CD147 Antibody

Pricing & Availability
Clone
HIM6 (See other available formats)
Regulatory Status
RUO
Workshop
VI N-L109
Other Names
Neurothelin, Basigin, EMMPRIN
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HIM6_PURE_CD147_Antibody_102804
Human peripheral blood lymphocytes stained with purified HIM6, followed by anti-mouse IgG FITC
  • HIM6_PURE_CD147_Antibody_102804
    Human peripheral blood lymphocytes stained with purified HIM6, followed by anti-mouse IgG FITC
  • HIM6_PURE_CD147_Antibody_IHC_072617
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for 5 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified human CD147 (Clone HIM6) antibody overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG antibody (Clone Poly4053) antibody (red) for an hour. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • HIM6_PURE_CD147_Antibody_IHC_072617
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for 5 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified human CD147 (Clone HIM6) antibody overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG antibody (Clone Poly4053) antibody (red) for an hour. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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306202 100 µg $114
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Description

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Capuchin Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBMCs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.5 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: inhibition of T cell activation2, immunohistochemical staining1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting1. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306221 and 306222).

Application References

(PubMed link indicates BioLegend citation)
  1. Menashi S, et al. 2003. Cancer Res. 63:7575. (WB IHC)
  2. Woodhead VE, et al. 2000. Int. Immunol. 12:1051. (Block)
  3. Reimers N, et al. 2004. Clin. Cancer Res. 10:3422. (IHC)
Product Citations
  1. Thornton N, et al. 2020. Nat Commun. 11:3569. PubMed
  2. Feliciano D, et al. 2021. Nat Commun. 12:4502. PubMed
  3. Etoh K, et al. 2019. J Cell Sci. 132:jcs226977. PubMed
  4. Satchwell TJ, et al. 2019. Nat Commun. 10:3806. PubMed
  5. Higashi S, et al. 2022. J Cell Sci. 135:. PubMed
  6. Aplin H 2015. Mol Hum Reprod. 21:81. PubMed
  7. Mohr T, et al. 2022. Front Oncol. 12:891511. PubMed
  8. Singh S, et al. 2021. Ocul Surf. S1542-0124:106. PubMed
  9. Ogura Y, et al. 2022. J Biol Chem. 298:102335. PubMed
  10. Eyster C, et al. 2011. Mol Biol Cell. 3.151388889. PubMed
  11. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
RRID
AB_314586 (BioLegend Cat. No. 306202)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
Distribution

Leukocytes, erythrocytes, platelets, endothelial cells

Function
Adhesion
Cell Type
Endothelial cells, Erythrocytes, Leukocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.

Gene ID
682 View all products for this Gene ID
UniProt
View information about CD147 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 05/09/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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