Purified anti-human CCX-CKR (CCRL1) Antibody

Pricing & Availability
Clone
13E11 (See other available formats)
Other Names
ACKR4, CC-CKR-11, VSHK1, PPR1, CCBP2, CCR10, CCR11, CKR-11, CCXCKR
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
13E11_Purified_CCRL1_Antibody_FC_022014.jpg
Human CCX-CKR (CCRL1)-transfected bovine coronary artery endothelial cell line (B300) was stained with purified anti-human CCRL1 (clone 13E11, filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
  • 13E11_Purified_CCRL1_Antibody_FC_022014.jpg
    Human CCX-CKR (CCRL1)-transfected bovine coronary artery endothelial cell line (B300) was stained with purified anti-human CCRL1 (clone 13E11, filled histogram) or purified mouse IgG2b, κ isotype control (open histogram), followed by anti-mouse IgG FITC.
Cat # Size Price Quantity Avail. Save
362102 100 µg $205
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Human CCX-CKR, also known as CCRL1, is a 40 kD, G-protein-coupled, seven transmembrane receptor. CCRL1 is a decoy receptor for CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25 that regulates chemotaxis of lymphocytes and cancer cells. Chemokine binding by CCX-CKR causes its internalization, reducing levels of available chemokine, and thus regulating migration of lymphocytes and cancerous cells. CCX-CKR is expressed by stromal cells of skin-draining lymph nodes, thymic epithelial cells, and immature dendritic cells (DCs). A low CCX-CKR expression in tumor cells correlates with a poor survival rate of breast cancer patients.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length CCX-CKR cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IP, Agonist - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation of CCX-CKR from cell lysates1, induction of CCX-CKR internalization1 and immunofluorescence1. 13E11 recognizes an epitope located within the first 14 amino acids of CCX-CKR.

Application References

(PubMed link indicates BioLegend citation)
  1. Takatsuka, S. et al. 2011. J. Pharmacol. Toxicol. Methods. 63:250. (IF, IP, Agonist)
Product Citations
  1. Bryce S, et al. 2016. J Immunol. 196: 3341-3353. PubMed
Publication Library
RRID
AB_2563201 (BioLegend Cat. No. 362102)

Antigen Details

Structure
G-protein-coupled seven transmembrane receptor, 40 kD.
Distribution

Stromal cells of skin-draining lymph nodes, thymic epithelial cells, immature DCs.

Function
C-C type chemokine scavenger and decoy receptor, reduces chemokine availability by sequestering them inside the cell.
Interaction
β-arrestin.
Ligand Receptor
CCL2, CCL8, CCL13, CCL19, CCL21, and CCL25.
Cell Type
Dendritic cells, Epithelial cells
Biology Area
Immunology
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Watts AO, et al. 2013. J. Biol. Chem. 288:7169. 
2. Comerford I, et al. 2010. Blood. 116:4130.
 

Gene ID
51554 View all products for this Gene ID
UniProt
View information about CCX-CKR (CCRL1) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/23/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account