Purified anti-human APRIL (TNFSF13, CD256) Antibody

Pricing & Availability
Clone
33H5 (See other available formats)
Other Names
A Proliferation Inducing Ligand (APRIL), TNFSF13, CD256, TALL2, TRDL1
Isotype
Mouse IgG2a, κ
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Cat # Size Price Quantity Avail. Save
524801 50 µg $85
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524802 500 µg $295
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Description

APRIL (A Proliferation-Inducing Ligand), also known as TNFSF13, stimulates cell proliferation. It is a member of the TNF superfamily of ligands, being most closely related to BAFF, with which it shares 30% amino acid sequence identity. APRIL is cleaved intracellularly by furin and is believed to exist mainly in a secreted, soluble form. As with most other TNF family members, APRIL exists as a functional homotrimer (total MW = 90 kD). It can bind to two cell-surface receptors: BCMA and TACI, which it shares with BAFF, to exert downstream T- and B-cell regulatory effects. It also possesses a heparin binding domain and has been demonstrated to bind to proteoglycans on the cell surface.

APRIL is most well known for its tumor proliferation effects. It is a potential biomarker, with serum levels elevated for certain cancers and expression levels elevated in cancer tissues. It is also a potential biomarker for certain autoimmune diseases. In fact, recombinant TACI has been brought to clinical trials as a neutralization drug against APRIL and BAFF for the treatment of systemic lupus erythematosus (SLE).

Besides forming homotrimers, APRIL can also form functional heterotrimers with BAFF. The stoichiometric relationship of the protomeric units is still unclear; however, it appears that these heterotrimers are significant in that they are elevated in the serum of certain autoimmune patients.

APRIL also forms an endogenous chimeric protein with another related protein, TWEAK, effectively called TWE-PRIL. It is believed that TWE-PRIL is membrane bound, and consists of the N-terminus of TWEAK with its transmembrane domain, and the C-terminus of APRIL with its receptor binding domain.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
His-cohesin-C-terminal APRIL ectodomain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For sandwich ELISA capture applications, a concentration range of 2-8 µg/ml is recommended when used in conjunction with biotinylated 53E11 (Cat. No. 524904) as the detection antibody, and recombinant human APRIL (TNFSF13) (ELISA Std.) (Cat. No. 591309) as the protein standard. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 33H5 reacts with recombinant APRIL homotrimer and recombinant APRIL-BAFF heterotrimer.

RRID
AB_2562633 (BioLegend Cat. No. 524801)
AB_2562634 (BioLegend Cat. No. 524802)

Antigen Details

Structure
Transmembrane protein of 26 kD, forms a homotrimer that is cleaved from the cell surface resulting in the soluble form of APRIL; forms heterotrimers with BLyS (BAFF)
Distribution
T cells, B cells, dendritic cells, monocytes, and macrophages
Function
Induces proliferation of T and B cells, promotes IgA class switching and downregulates T-cell dependent antibody responses, regulates the growth of a variety of tumor cells
Ligand Receptor
TNFRSF13b (TACI), TNFRSF17 (BCMA)
Cell Type
T cells, B cells, Dendritic cells, Monocytes, Macrophages
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hymowitz, S, et al. 2005. J. Biol. Chem. 280:7218.
2. Ingold K, et al. 2005. J. Exp. Med. 201:1375.
3. Good K, et al. 2009. J. Immunol. 182:890.
4. Bossen C, et al. 2008. Blood 111:1004.
5. Bossen C, et al. 2006. J. Biol. Chem. 281:13964.
6. Pradet-Balade B, et al. 2002. EMBO J. 21:5711.
7. Hahne M, et al. 1998. J. Exp. Med. 188:1185.
8. Medema JP, et al. 2003. Cell Death Differ. 10:1121.
9. Planelles L, et al. 2007. Haematologica. 92:1284.
10. Dillon SR, et al. 2010. Arthritis Res. Ther 12:R48.
11. Wallweber H, et al. 2004. J. Mol. Biol. 343:283.
12. Chen Y, et al. 2011. PLoS ONE 6:e22202.
13. Roschke V, et al. 2002. J. Immunol. 169:4314.

Gene ID
8741 View all products for this Gene ID
UniProt
View information about APRIL on UniProt.org

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