Purified anti-HSC70 (HSPA8) Antibody

Pricing & Availability
Clone
9/2 (See other available formats)
Other Names
Heat Shock 70 kD Protein 8, HSP70, HSP73, HSPA10, Heat Shock Cognate Protein 54, HSC54, heat stress cognate 70
Isotype
Mouse IgG2b
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Product Citations
publications
9slash2_PURE_HSC70_HSPA8_Antibody_WB_122315
Total lysates (15 µg protein) from 293T and 293T/HSC70 CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-HSC70 (HSPA8) antibody, clone 9/2 (upper) or 1:3000 diluted anti-GAPDH (poly6314, Cat. No. 631401) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) for anti-HSC70 (HSPA8) antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat. No. 406401) for GAPDH. Lane M: MW ladder.
  • 9slash2_PURE_HSC70_HSPA8_Antibody_WB_122315
    Total lysates (15 µg protein) from 293T and 293T/HSC70 CRISPR/Cas9 knockdown (KD) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-HSC70 (HSPA8) antibody, clone 9/2 (upper) or 1:3000 diluted anti-GAPDH (poly6314, Cat. No. 631401) antibody (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) for anti-HSC70 (HSPA8) antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat. No. 406401) for GAPDH. Lane M: MW ladder.
  • 9slash2_PURE_HSC70_HSPA8_Antibody_WB_2_122315
    Western blot analysis of cell lysates from HeLa (lane 1), THP-1 (lane 2), HEK293 (lane 3), and Raw264.7 (lane 4). Anti-HSC70 antibody (clone 9/2) was used and followed by HRP anti-mouse IgG antibody. GAPDH antibody (clone poly6314) was used as a loading control.
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682102 100 µg $225
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Description

Heat shock cognate 71 kD protein or HSC70 (HSPA8) is one of the major constitutively expressed, cognate protein chaperones to supply a multitude of the housekeeping chaperoning functions. As a chaperone, HSC70 binds to nascent polypeptides to facilitate correct folding. Besides the chaperone functions, secreted HSC70 is involved in inflammatory signal pathways via extracellular interaction with TLR2/TLR4. It also plays a central role in modulating antigen transport within cells to control MHC class II presentation during nutrient stress. Furthermore, HSC70 may serve as a molecular switch to modulate endocytic and autophagy pathways.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human HSC70 fragment (1-1755bp of Acc. Y00371)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566574 (BioLegend Cat. No. 682102)

Antigen Details

Structure
646 amino acids with a predicted molecular weight of approximately 71 kD.
Distribution

Cytoplasm, nucleus, and cell membrane.

Function
Chaperone and antigen transport.
Interaction
PACRG, HSPH1/HSP105, IRAK1BP1, BAG1, DNAJC7, DNAJB14, E3 ligase CHIP, CITED1 , CDC5L, PLRG1 and BCAS2, SV40 VP1, TRIM5, METTL21A, PARK2, and FOXP3.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Signal Transduction, Transcription Factors
Molecular Family
Protein Kinases/Phosphatase, Heat Shock Proteins
Antigen References

1. Deffit SN, Blum JS. 2015. Mol. Immunol. 68:85-8.
2. Pratt WB, et al. 2015. Annu. Rev. Pharmacol. Toxicol. 55:353.
3. Stricher F, et al. 2013. Autophagy 9:1937.
4. Liao Y, et al. 2014. Curr. Pharm. Des. 20:101.
5. Höhfeld J. 1998. Biol. Chem. 379:269.

Gene ID
3312 View all products for this Gene ID
UniProt
View information about HSC70 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/18/2017

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