- 6F12-H4 (See other available formats)
- Regulatory Status
- Other Names
- Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
Covance Catalog# MMS-5079
- Mouse IgG1
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- Product Citations
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Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.Product Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Host Species
- This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, tri-methylated at Lysine-9.
- Phosphate-buffered solution.
- The antibody was purified by affinity chromatography.
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100 - 1:1000*
• IHC: 1:50 - 1:100
Tissue: Frozen sections
Primary Incubation: 24 hours at 4°C
- Application Notes
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).
* Expected MW: 17 kD
WB Positive Control: HeLa cell lysate
IHC Positive Control: Rat Brain
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
AB_2564794 (BioLegend Cat. No. 815601)
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction
- Gene ID
- 8350 View all products for this Gene ID
- View information about Histone H3 Trimethyl Lys9 on UniProt.org
|Purified anti-Histone H3 Trimethyl (Lys9)||6F12-H4||WB, IHC-F|
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