Purified anti-Histone H3 Trimethyl (Lys9) Antibody (Previously Covance catalog# MMS-5079)

Pricing & Availability
Clone
6F12-H4 (See other available formats)
Other Names
Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
Previously
Covance Catalog# MMS-5079
Isotype
Mouse IgG1
Ave. Rating
Submit a Review
Product Citations
publications
6F12-H4_Purified_Histone_H3_Trimethyl_Lys9_Antibody_IHC_030315
Staining of Clone 6F12-H4 on frozen rat brain.
  • 6F12-H4_Purified_Histone_H3_Trimethyl_Lys9_Antibody_IHC_030315
    Staining of Clone 6F12-H4 on frozen rat brain.
Cat # Size Price Quantity Avail. Save
815601 100 µl $285
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, tri-methylated at Lysine-9.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IHC

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:100 - 1:1000*
IHC: 1:50 - 1:100

Tissue: Frozen sections
Pretreatment: None
Primary Incubation: 24 hours at 4°C

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

* Expected MW: 17 kD
WB Positive Control: HeLa cell lysate
IHC Positive Control: Rat Brain

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2564794 (BioLegend Cat. No. 815601)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Signal Transduction
Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone H3 Trimethyl (Lys9) on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/14/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account