Purified anti-Histone H2B Antibody

Pricing & Availability
Clone
W17025A (See other available formats)
Other Names
Histone Cluster 1, H2bb, H2B Histone Family-Member F, H2BFF, Histone H2B.1, H2B/F
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W17025A_PURE_Histone_H2B_Antibody_WB_011718
Total cell lysates (15 µg protein) from HeLa (lane 1), HaCaT (lane 2), and NIH/3T3 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with the indicated concentration (µg/mL) of purified anti-Histone H2B (Clone W17025A) or a competitor’s clone used at the manufacturer’s recommended concentration (far right). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) for clone W17025A and HRP goat anti-mouse IgG (Cat. No. 405306) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular weight ladder.
  • W17025A_PURE_Histone_H2B_Antibody_WB_011718
    Total cell lysates (15 µg protein) from HeLa (lane 1), HaCaT (lane 2), and NIH/3T3 cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with the indicated concentration (µg/mL) of purified anti-Histone H2B (Clone W17025A) or a competitor’s clone used at the manufacturer’s recommended concentration (far right). Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) for clone W17025A and HRP goat anti-mouse IgG (Cat. No. 405306) for the competitor’s clone, each at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular weight ladder.
  • W17025A_PURE_Histone_H2B_Antibody_WB_2_081619
    Western blot of purified anti-Histone H2B antibody (clone W17025A). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.25 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
606301 25 µg $85
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606302 100 µg $215
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Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. Histones play central roles in transcription regulation, DNA repair, DNA replication and chromosomal stability. There are many variants of histones H2A and H2B. Specific H2A and H2B variants may preferentially associate with each other resulting in different combinations of variants and leading to the increased combinatorial complexity of nucleosomes. When being catalyzed by a series of histone modifying enzymes, histones may undergo various post-translational modifications such as acetylation, methylation, phosphorylation, ubiquitylation and SUMOylation. The dysregulation of histone modifying enzymes will alter the histone post-modification patterns and cause diverse diseases including cancers.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Drosophila
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human H2B1A peptide (111-127 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per ml (1:250-2000). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Reactivity to Drosophila was only validated with the purified format.

RRID
AB_2728464 (BioLegend Cat. No. 606301)
AB_2728465 (BioLegend Cat. No. 606302)

Antigen Details

Structure
126 amino acids with a predicted molecular weight of approximately 14kD but observed at approximately 17kD as the highly positively charged Histone protein tends to migrate slower on SDS-PAGE gel.
Distribution

Eukaryotic cells and nucleus.

Function
Histone H2B is one of the building blocks of nucleosome. It plays a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction
Histone H2A
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication
Antigen References

1. Ma F, Zhang CY. 2016. Expert Rev. Mol. Diagn. 16:297.
2. Li J, et al. 2016. Biomed. Rep. 4:293.
3. Shaytan AK, et al. 2015. Curr. Opin. Struct. Biol. 32:48.
4. Wright DE, Kao CF. 2015. Epigenetics 10:122.
5. Kim J, et al. 2009. Cell. 137:459.
6. Cheung WL, et al. 2003. Cell. 113:507.

Gene ID
3018 View all products for this Gene ID
UniProt
View information about Histone H2B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/20/2019

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This data display is provided for general comparisons between formats.
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