- Clone
- W15090A (See other available formats)
- Other Names
- Histone deacetylase-like 1 (HDACL1), MRXS6, Wilson-Turner X-linked mental retardation syndrome (WTS), RPD3
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total lysates (10µg protein) from HeLa and HeLa HDAC8 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-HDAC8 antibody , clone W15090A (upper) or 1:3000 diluted anti-GAPDH (poly6314, cat#631401) antibody (lower). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306) for the anti-HDAC8 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat# 406401) for GAPDH. Lane M is the Molecular weight ladder. -
Total lysates (15 µg protein) mouse brain (lane 1) and HeLa cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-HDAC8 Antibody, clone W15090A (upper) or 1:3000 diluted Purified anti-GAPDH Antibody, clone poly6314 (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-HDAC8 Antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody. -
HeLa cells were fixed with 2% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2 µg/ml purified anti-human HDAC8 (clone W15090A) antibody for one hour, followed by staining with Alexa Fluor® 594 anti-mouse IgG antibody (red), Alexa Fluor® 488 Phalloidin (green), and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 685502 | 100 µg | $265 | ||||
Histone deacetylases (HDACs) are a family of enzymes responsible for the removal of acetyl groups from lysine residues on the core histones and other non-histone proteins. HDAC8 is a type I histone deacetylase and was initially found to deacetylate a variety of acetylated histone variants in vitro. HDAC8 is a kinectically and structurally well-characterized deacetylase that was reported to catalyze several nuclear proteins, which include ERR-α, hEST1B, and SMC3. Patients with HDAC8 mutations show Cornelia de Lange Syndrome (CdLS) and Wilson-turner Syndrome (WTS) phenotypes. Elevated expression of HDAC8 has been observed in various tumor types, such as acute lymphoblastic leukemia (AML), neuroblastomas, and glimoas. HDAC8 is implicated as a therapeutic target in cancer and infectious diseases.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human HDAC8 recombinant protein expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
KO/KD-WB, ICC - Validated - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2572204 (BioLegend Cat. No. 685502)
Antigen Details
- Structure
- 377 amino acids with a predicted molecular weight of 41.8 kD. It belongs to the type I Histone deacetylase subfamily.
- Distribution
-
Nucleus and cytoplasm.
- Function
- HDAC8 is a deacetylase and regulates gene expression through catalyzing the removal of acetyl groups on lysine residues in the core histones and transcriptional factors.
- Interaction
- Interacts with PEPB2-MYH11, CBFA2T3, SMG5, and alpha-SMA.
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
- Antigen References
-
1. Qi J, et al. 2015. Cell Stem Cell. 17:597.
2. Tian Y, et al. 2015. Cancer Res. 75:4803.
3. Feng L, et al. 2015. J. Hum. Genet. 60:165.
4. Ha SD, et al. 2014. J. Immunol. 193:1333.
5. Higuchi T, et al. 2013. Blood 121:3640.
6. Deardorff MA, et al. 2012. Nature 489:313. - Gene ID
- 55869 View all products for this Gene ID
- UniProt
- View information about HDAC8 on UniProt.org
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