Purified anti-HDAC8 Antibody

Pricing & Availability
Clone
W15090A (See other available formats)
Other Names
Histone deacetylase-like 1 (HDACL1), MRXS6, Wilson-Turner X-linked mental retardation syndrome (WTS), RPD3
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_W15090A_Pure_HDAC8_Antibody_WB_110117
Total lysates (10µg protein) from HeLa and HeLa HDAC8 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-HDAC8 antibody , clone W15090A (upper) or 1:3000 diluted anti-GAPDH (poly6314, cat#631401) antibody (lower). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306) for the anti-HDAC8 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat# 406401) for GAPDH. Lane M is the Molecular weight ladder.
  • 1_W15090A_Pure_HDAC8_Antibody_WB_110117
    Total lysates (10µg protein) from HeLa and HeLa HDAC8 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti-HDAC8 antibody , clone W15090A (upper) or 1:3000 diluted anti-GAPDH (poly6314, cat#631401) antibody (lower). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306) for the anti-HDAC8 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP (Cat# 406401) for GAPDH. Lane M is the Molecular weight ladder.
  • 2_W15090A_Pure_HDAC8_Antibody_1_WB_033016
    Total lysates (15 µg protein) mouse brain (lane 1) and HeLa cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) Purified anti-HDAC8 Antibody, clone W15090A (upper) or 1:3000 diluted Purified anti-GAPDH Antibody, clone poly6314 (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-HDAC8 Antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody.
  • 3_W15090A_Pure_HDAC8_Antibody_2_ICC_041519
    HeLa cells were fixed with 2% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2 µg/ml purified anti-human HDAC8 (clone W15090A) antibody for one hour, followed by staining with Alexa Fluor® 594 anti-mouse IgG antibody (red), Alexa Fluor® 488 Phalloidin (green), and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
685502 100 µg $265
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Description

Histone deacetylases (HDACs) are a family of enzymes responsible for the removal of acetyl groups from lysine residues on the core histones and other non-histone proteins. HDAC8 is a type I histone deacetylase and was initially found to deacetylate a variety of acetylated histone variants in vitro. HDAC8 is a kinectically and structurally well-characterized deacetylase that was reported to catalyze several nuclear proteins, which include ERR-α, hEST1B, and SMC3. Patients with HDAC8 mutations show Cornelia de Lange Syndrome (CdLS) and Wilson-turner Syndrome (WTS) phenotypes. Elevated expression of HDAC8 has been observed in various tumor types, such as acute lymphoblastic leukemia (AML), neuroblastomas, and glimoas. HDAC8 is implicated as a therapeutic target in cancer and infectious diseases.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human HDAC8 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2572204 (BioLegend Cat. No. 685502)

Antigen Details

Structure
377 amino acids with a predicted molecular weight of 41.8 kD. It belongs to the type I Histone deacetylase subfamily.
Distribution

Nucleus and cytoplasm.

Function
HDAC8 is a deacetylase and regulates gene expression through catalyzing the removal of acetyl groups on lysine residues in the core histones and transcriptional factors.
Interaction
Interacts with PEPB2-MYH11, CBFA2T3, SMG5, and alpha-SMA.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Qi J, et al. 2015. Cell Stem Cell. 17:597.
2. Tian Y, et al. 2015. Cancer Res. 75:4803.
3. Feng L, et al. 2015. J. Hum. Genet. 60:165.
4. Ha SD, et al. 2014. J. Immunol. 193:1333.
5. Higuchi T, et al. 2013. Blood 121:3640.
6. Deardorff MA, et al. 2012. Nature 489:313.

Gene ID
55869 View all products for this Gene ID
UniProt
View information about HDAC8 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 11/01/2017

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