Purified anti-HDAC1 Phospho (Ser406) Antibody

Pricing & Availability
Clone
BT-15 (See other available formats)
Other Names
Histone Deactylase 1, RPD3L1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
BT-15_Pure_HDAC1-phospho_Antibody_1_012319
Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 300 ng/mL nocodazole for 16 hours were resolved on a 4-20% Tris-glycine gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-HDAC1 Phospho (Ser406) Antibody, clone BT-15, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat Anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Equal HDAC1 loading was confirmed by probing membranes with anti-HDAC1 antibody, clone Poly6074 (Cat. No. 607401) at a 1:1000 dilution. Lane M: Molecular Weight marker.
  • BT-15_Pure_HDAC1-phospho_Antibody_1_012319
    Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated (+) with 300 ng/mL nocodazole for 16 hours were resolved on a 4-20% Tris-glycine gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-HDAC1 Phospho (Ser406) Antibody, clone BT-15, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat Anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Equal HDAC1 loading was confirmed by probing membranes with anti-HDAC1 antibody, clone Poly6074 (Cat. No. 607401) at a 1:1000 dilution. Lane M: Molecular Weight marker.
  • BT-15_Pure_HDAC1-phospho_Antibody_2_012319
    Untreated HELA cells (left) and HELA cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with Purified anti-HDAC1 Phospho (Ser406) Antibody (clone BT-15) followed by PE Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) and DAPI.
  • BT-15_Pure_HDAC1-phospho_Antibody_3_012419_updated.png
    Untreated HELA cells (left) and HELA cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with Purified anti-HDAC1 Phospho (Ser406) Antibody (clone BT-15) followed by Goat anti-mouse IgG (minimal x-reactivity) Antibody (clone Poly4053) PE and anti-Histone H3-Phosphorylated (Ser28) Antibody (clone HTA28) Alexa Fluor® 647.
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611001 25 µg $95
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611002 100 µg $225
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Description

Histone Deacetylase 1 (HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and differentiation. The enzyme functions by removing acetyl moieties from histone targets, resulting in histone compaction and alterations in nucleosomal positioning.  Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. This modification plays an essential role in regulating cell cycle progression, as well as controlling the expression of genes involved in central nervous system development.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Zebrafish2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site.

This clone recognizes zebrafish HDAC1 phosphorylated at Ser4062.

Application References

(PubMed link indicates BioLegend citation)
  1. Segre CV, et al, 2016. mAbs. 8: 37-42
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.

Antigen Details

Structure
HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
Distribution

Nucleus/Ubiquitously expressed

Function
Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References
  1. Segre CV, et al, 2016. mAbs. 8: 37-42.
  2. Loponte S, et al, 2016. Sci Rep, 6: 30213.
Gene ID
3065 View all products for this Gene ID
UniProt
View information about HDAC1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 01/23/2019

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