Purified anti-HDAC1 Antibody (Previously Covance catalog# MMS-5071)

Pricing & Availability
Clone
10E2/HDAC1 (See other available formats)
Other Names
Histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1
Previously
Covance Catalog# MMS-5071
Isotype
Mouse IgG1
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Product Citations
publications
10E2slashHDAC1_PURE_HDAC1_Antibody_IHC_053018
IHC staining of purified anti-HDAC1 antibody (clone 10E2/HDAC1) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 10E2slashHDAC1_PURE_HDAC1_Antibody_IHC_053018
    IHC staining of purified anti-HDAC1 antibody (clone 10E2/HDAC1) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 2_10E2slashHDAC1_PURE_HDAC1_Antibody_WB_2_120117
    Total cell lysates (15 µg protein) from HeLa (lanes marked as 1) and NIH3T3 cells (lanes marked as 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to a nitrocellulose membrane, and probed with serial dilutions of purified anti-HDAC1 antibody (clone 10E2/HDAC1) as indicated (1:500, 1 µg/mL; 1:1000, 0.5 µg/mL; 1:2000, 0.25 µg/mL; 1:4000, 0.125 µg/mL). Proteins were visualized using chemiluminescence detection after incubation with a 1:3000 dilution of HRP conjugated goat anti-mouse-IgG secondary antibody for the anti-HDAC1 (upper panel) or a 1:5000 dilution of Direct-Blot™ HRP anti-β-Actin (clone 2F1-1, lower panel). Lane M: Molecular weight ladder. Non-specific bands can be detected at high antibody concentrations.
  • 3_10E2slashHDAC1_PURE_HDAC1_Antibody_WB_3_113018
    Total cell lysates (10 µg total protein) from HeLa (WT) and HeLa HDAC1 CRISPR/CAS9 knockout (KO) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) of Purified anti- HDAC1 Antibody, clone 10E2/HDAC1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
815101 100 µl $234
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Description

HDAC1 (histone deacetylase 1) is a 65 kD member of the Class I histone deacetylase family, which is similar to the yeast RPD3 protein. This nuclear protein operates in concert with histone acetyltransferases to control core histone acetylation in highly conserved lysine residues. HDAC activity has been associated with transcriptional repression and nucleosomal condensation. HDAC1 deacetylates RELA at residue K310, which results in the inhibition of NF-κB activity. HDAC1 has been suggested to participate in the regulation of eukaryotic gene expression and cell cycle progression. HDAC1-deficient mice were shown to be embryonic lethal, suggesting that HDAC1 plays a crucial role during embryonic development.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the C-terminus of the human HDAC1 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB, KO/KD-WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration of 10 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

RRID
AB_2564789 (BioLegend Cat. No. 815101)

Antigen Details

Structure
Expected MW: 60 kD
Biology Area
Cell Biology, Neuroscience
Gene ID
3065 View all products for this Gene ID
UniProt
View information about HDAC1 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 04/04/2019

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This data display is provided for general comparisons between formats.
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