Purified anti-Granzyme B Antibody

Pricing & Availability
Clone
O94E6/GZMB (See other available formats)
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase, CSPB, CTLA-1, CGL-1, CGL1
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
O94E6-GZMB_Purified_Granzyme B_Antibody_1WB_112514
Western blot analysis of 50ng of recombinant human Granzyme B (lane 1), Daudi (lane 2), Jurkat (lane 3), and PBMC (lane 4) using anti-Granzyme B antibody (clone O94E6/GZMB). Anti-GAPDH antibody was used as a loading control.
  • O94E6-GZMB_Purified_Granzyme B_Antibody_1WB_112514
    Western blot analysis of 50ng of recombinant human Granzyme B (lane 1), Daudi (lane 2), Jurkat (lane 3), and PBMC (lane 4) using anti-Granzyme B antibody (clone O94E6/GZMB). Anti-GAPDH antibody was used as a loading control.
  • O94E6-GZMB_Purified_Granzyme B_Antibody_2WB_112514
    Western blot analysis of PBMC (lane 1), PBMC activated with CD3 and CD28 antibody for two days (lane 2), and mouse thymus (lane 3) using anti-Granzyme B antibody(clone O94E6/GZMB ). Anti-β actin antibody was used as a loading control.
Cat # Size Price Quantity Avail. Save
674301 25 µg $85
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674302 100 µg $255
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Description

Granzyme B is a serine protease that is produced mainly by cytotoxic T cells (CTL) and natural killer (NK) cells. It plays an important role in cytotoxic lymphocyte-mediated apoptosis, chronic inflammation, and impaired wound healing. Granzyme B is packaged in cytoplasmic granules that are exocytosed towards a bound target cell, which subsequently activates multiple protein substrates that induce apoptosis. Perforin facilitates the transfer of Granzyme B into the target cell. Most circulating CD56+, CD8-, NK cells, and approximately half of circulating CD8+ T cells coexpress both Granzyme A and B. The activation of CD8+ and CD4+ T lymphocytes induce substantial expression of Granzyme B, but not Granzyme A. Recently, Granzyme B has been found to be produced by other cell types such as CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, B cells, and smooth muscle cells. Elevated levels of Granzyme B are also found in some kinds of autoimmune diseases, type 1 diabetes, and cardiovascular diseases.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment corresponding to amino acids 21-247 of Human Granzyme B produced in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted at 4°C
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564582 (BioLegend Cat. No. 674301)
AB_2564583 (BioLegend Cat. No. 674302)

Antigen Details

Structure
247 amino acids with a molecular weight of approximately 28 kD.
Distribution

Cytotoxic T cells, Natural killer (NK) cells, and neutrophils.

Function
Induces target cell apoptosis by activating caspase independent pathways and is induced in CD8+ T lymphocytes with ConA / IL-2 and CD4+ lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Targets of CTL and NK cells.
Cell Type
T cells, NK cells, Neutrophils, B cells, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Edwards KM, et al. 1999. J. Biol. Chem. 274:30468.
2. Grossman WJ, et al. 2004. Blood 104:2840.
3. Heusel JW, et al. 1994. Cell 76:977.
4. Schmid J and Weissmann C. 1987. J. Immunol. 1:250.
5. Trapani JA, et al. 1988. Proc. Natl. Acad. Sci. USA 85:6934.
6. Hiebert PR and Granville DJ. 2012. Trends Mol. Med. 12:732.
7. Saito Y, et al. 2011. J. Cardiol. 57:141.
8. Hiebert PR, et al. 2012. Trends Mol. Med. 18:732.
9. Ewen CL, et al. 2012. Cell Death Differ. 1:28.

Gene ID
3002 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 12/01/2014

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