Purified anti-Glu-Glu Epitope Tag Antibody (Previously Covance catalog# MMS-115P)

Pricing & Availability
Clone
Glu-Glu (See other available formats)
Other Names
MPyVgp2, Polyomavirus medium t antigen, Polyomavirus middle t antigen, EYMPME
Previously
Covance Catalog# MMS-115P
Isotype
Mouse IgG1
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Product Citations
publications
Glu-Glu_PURE_Glu-Glu_Epitope_Tag_WB_060418
Total lysates (15 µg protein) from 293E transfected with or without Glu-glu tagged plasmid were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 (1 µg/ml) purified anti Glu-Glu antibody, clone Glu-Glu. Proteins were visualized using chemiluminescence detection by incubation with and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
  • Glu-Glu_PURE_Glu-Glu_Epitope_Tag_WB_060418
    Total lysates (15 µg protein) from 293E transfected with or without Glu-glu tagged plasmid were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 (1 µg/ml) purified anti Glu-Glu antibody, clone Glu-Glu. Proteins were visualized using chemiluminescence detection by incubation with and HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 664804, 1:25,000 dilution).
Cat # Size Price Quantity Avail. Save
901801 200 µl $303
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901802 500 µl $474
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Description

The Glu-Glu tag consists of the peptide sequence, CEEEEYMPME, which is derived from the polyoma virus medium T antigen. This tag is often used as a protein modification in order to simplify the labeling and detection of proteins. This unique amino acid sequence allows for specific antibody detection in western blotting and other immunostaining techniques. Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins. This antibody clone is capable of recognizing both EYMPME and EFMPME sequences.

Product Details
Technical data sheet

Product Details

Reactivity
EYMPME and EFMPME Tags
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The Glu-Glu monoclonal antibody was raised against the peptide sequence CEEEEYMPME derived from the polyoma virus medium T antigen.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, IP, IF, Purification

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition. This antibody is sold for laboratory research use only, not for human or in-vivo use. BioLegend antibodies may not be resold or modified for resale without prior written approval.
WB: 1:1,000*
IF: 1:1,000
IP: 1:150

Application Notes

This antibody is effective in immunofluorescence, immunoblotting, and immunoprecipitation of tagged proteins.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

It is extremely specific and recognizes either six amino acid sequence, EYMPME, or EFMPME.

Application References

(PubMed link indicates BioLegend citation)
  1. Wells CD, et al. 2001. J. Biol. Chem. 276:28897.
  2. Kim M, et al. 2001. J. Biol. Chem. 276:23262.
  3. Grussenmeyer T, et al. 1985. Proc. Nat. Acad. Sci. USA. 82:7952.
  4. Pinkas J, et al. 2002. Cancer Res. 62:4781. (WB, IF) PubMed
  5. Jeon JP, et al. 2012. J. Biol. Chem. 287:17029. (WB)
Product Citations
  1. Leder J 2002. Cancer Res. 62:4781-4790. PubMed
Publication Library
RRID
AB_2565011 (BioLegend Cat. No. 901801)
AB_2565012 (BioLegend Cat. No. 901802)

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Go To Top Version: 7    Revision Date: 01/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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