Purified anti-GFP Antibody

Pricing & Availability
Clone
FM264G (See other available formats)
Other Names
Green Fluorescent Protein
Isotype
Rat IgG2a, κ
Ave. Rating
1 reviews
Product Citations
publications
FM264G_Purified_GFP_Antibody_120618
GFP-transfected CHO cells were fixed, permeabilized, and intracellularly stained with anti-GFP (clone FM264G) Purified (filled histogram) or rat IgG2a, κ isotype control (open histogram) followed by anti-rat IgG PE.
  • FM264G_Purified_GFP_Antibody_120618
    GFP-transfected CHO cells were fixed, permeabilized, and intracellularly stained with anti-GFP (clone FM264G) Purified (filled histogram) or rat IgG2a, κ isotype control (open histogram) followed by anti-rat IgG PE.
Cat # Size Price Quantity Avail. Save
338001 25 µg $60
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338002 100 µg $110
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Description

Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea Victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Product Details
Technical data sheet

Product Details

Reactivity
GFP-tagged proteins
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TLR9-GFP transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
ICC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry1.

Application References

(PubMed link indicates BioLegend citation)
  1. Stephen LA, et al. 2018. Dev. Cell. 47(1):122-132.e4. PubMed (ICC)
Product Citations
  1. Chen G, et al. 2011. J Virol. 85:11131. PubMed
  2. Luo Y, et al. 2012. J Control Release. 162:28. PubMed
  3. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  4. Wroblewska A 2018. Cell. 175:1141. PubMed
Publication Library
RRID
AB_1279415 (BioLegend Cat. No. 338001)
AB_1279414 (BioLegend Cat. No. 338002)

Antigen Details

Biology Area
Cell Biology, Immunology
Antigen References

1. Ishikura H, et al. 2004. Anticancer Res. 24:719.
2. Rizzuto R, et al. 1996. Curr. Biol. 6:183.
3. Chalfie M, et al. 1994. Science 263:802.

Gene ID
NA
UniProt
View information about GFP on UniProt.org

Reviews

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Related FAQs

Can I use common compensation control for GFP, CFSE and FITC because they emit in the same channel?
It is not recommended even if they emit in the same channel because these are still different fluors with different brightness intensities. Individual compensation controls should be employed.
Go To Top Version: 3    Revision Date: 12/05/2018

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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