Purified anti-GFAP Antibody (Previously Covance catalog# PCK-591P)

Pricing & Availability
Clone
Poly28294 (See other available formats)
Other Names
Glial fibrillary acidic protein
Previously
Covance Catalog# PCK-591P
Isotype
Chicken Polyclonal IgY
Ave. Rating
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Product Citations
publications
Poly28294_Purified_GFAP_Antibody_1_IHC_022415
Staining of GFAP on formalin-fixed paraffin embedded Alzheimer's diseased brain tissue.
  • Poly28294_Purified_GFAP_Antibody_1_IHC_022415
    Staining of GFAP on formalin-fixed paraffin embedded Alzheimer's diseased brain tissue.
  • Poly28294_Purified_GFAP_Antibody_2_IF_012615
    Rat glial cells in mixed culture stained with affinity purified GFAP (red) Ck PAb. Nuclei stained with Hoechst.
See high resolution IHC data...
Cat # Size Price Quantity Avail. Save
829401 100 µl $423
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Description

Glial fibrillary acidic protein (GFAP) is the 10nm or intermediate filament protein found specifically in astrocytic cells in the central nervous system. Antibodies to GFAP are therefore widely used to identify astrocytic cells in situ and in tissue culture. This antibody was developed using bovine spinal cord GFAP purified from a Triton X-100 extract of myelin associated material and further purified by centrifugation and ion exchange chromatography in 6m urea on DEAE cellulose.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse, Cow, Pig, Chicken
Antibody Type
Polyclonal
Host Species
Chicken
Immunogen
Native GFAP purified from bovine spinal cord.
Formulation
Phosphate-buffered solution, containing 0.2% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC, IF, WB

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:10,000 - 50,000
IF: 1:1,000 - 5,000
IHC: 1:1,000 - 5,000

Tissue: Formalin-fixed Paraffin-embedded human brain
Retrieval: None required
Primary incubation: 20 minutes

Application Notes

The antibody is effective in immunofluorescence (IF), Western Blot (WB), and immunohistochemistry (IHC).

Application References

(PubMed link indicates BioLegend citation)
  1. Sosunov A, Guilfoyle E, Wu X, McKhann G, Goldman J. Phenotypic Conversions of "Protoplasmic" to "Reactive" Astrocytes in Alexander Disease. J. Neurosci.; 33: 7439 - 7450, Apr 2013. [IF] PubMed
Product Citations
  1. Sosunov A, et al. 2013. J Neurosci. 33:7439-7450. PubMed
Publication Library
RRID
AB_2564929 (BioLegend Cat. No. 829401)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 12/13/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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