- Poly28294 (See other available formats)
- Other Names
- Glial fibrillary acidic protein
Covance Catalog# PCK-591P
- Chicken Polyclonal IgY
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- Product Citations
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Glial fibrillary acidic protein (GFAP) is the 10nm or intermediate filament protein found specifically in astrocytic cells in the central nervous system. Antibodies to GFAP are therefore widely used to identify astrocytic cells in situ and in tissue culture. This antibody was developed using bovine spinal cord GFAP purified from a Triton X-100 extract of myelin associated material and further purified by centrifugation and ion exchange chromatography in 6m urea on DEAE cellulose.Product Details
- Human, Rat, Mouse, Cow, Pig, Chicken
- Antibody Type
- Host Species
- Native GFAP purified from bovine spinal cord.
- Phosphate-buffered solution, containing 0.2% sodium azide.
- The antibody was purified by affinity chromatography.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
IHC, IF, WB
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemistry.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:10,000 - 50,000
• IF: 1:1,000 - 5,000
• IHC: 1:1,000 - 5,000
Tissue: Formalin-fixed Paraffin-embedded human brain
Retrieval: None required
Primary incubation: 20 minutes
- Application Notes
The antibody is effective in immunofluorescence (IF), Western Blot (WB), and immunohistochemistry (IHC).
(PubMed link indicates BioLegend citation)
- Sosunov A, Guilfoyle E, Wu X, McKhann G, Goldman J. Phenotypic Conversions of "Protoplasmic" to "Reactive" Astrocytes in Alexander Disease. J. Neurosci.; 33: 7439 - 7450, Apr 2013. [IF] PubMed
- Product Citations
AB_2564929 (BioLegend Cat. No. 829401)
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